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Defining the immune mechanism with monoclonal antibodies

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Summary

It has long been realized that only the study of homogeneous antibodies or cell populations could enable a definitive understanding of much of the immune mechanism. Hybridoma technology has greatly facilitated such approaches. Hybridoma antibodies have been used to delineate both B cell and T cell subpopulations. T cell studies per se have been accomplished by the use of T cell hybridoma cell lines producing a variety of factors. Anti-idiotypes against B cell hybridoma antibodies have been used to characterize T cell receptors and factors. B cell studies have been facilitated by hybridomas that have made available the immunoglobulin of pre-B cells or defective B cell lines. Hybridoma antibodies have also been used to dissect closely related antibody families and the potential for responsiveness against a variety of antigenic determinants. Finally, hybridomas have provided a primary source of material for protein and DNA sequence analysis. In our laboratories hybridoma antibodies derived against the murine H-2 locus have demonstrated the ability of B cell antibodies to discriminate amongst H2 mutants—a capacity previously attributed only to T cell specificities. Hybridoma antibodies have also been generated by fusions with antigen stimulated neonatal B cells to provide homogeneous antibodies reflective of the earliest developmental immunoglobulin readout. Such probes should increase our understanding of the processes involved in the generation of both the T and B cell repertoires.

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Authors and Affiliations

  1. Department of Immunopathology, Scripps Clinic and Research Foundation, 10666 North Torrey Pines Road, 92037, La Jolla, California

    Norman R. Klinman, Kathleen A. Denis & Linda A. Sherman

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  1. Norman R. Klinman
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  2. Kathleen A. Denis
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  3. Linda A. Sherman
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Additional information

This work was supported by United States Public Health Service Grants AI 15797 and AI 15710. This symposium was supported in part by the following organizations: Bethesda Research Laboratories, Cetus Corporation, Hybritech Incorporated, MAB-Monoclonal Antibodies, Inc., National Capital Area Branch of the Tissue Culture Association, New England Nuclear Corporation, and Ortho Pharmaceutical Corporation.

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Klinman, N.R., Denis, K.A. & Sherman, L.A. Defining the immune mechanism with monoclonal antibodies. In Vitro 17, 1029–1035 (1981). https://doi.org/10.1007/BF02618600

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