Summary
A unique population of human bone marrow-derived, adherent fibroblastlike cells differentiates to adipocyte morphology when grown in vitro in the presence of horse serum and hydrocortisone sodium hemisuccinate. Over the initial 8-weeks growth at 37°C, 7% CO2, these cells accumulate Oil Red O-positive lipid and form colonies of over 100 cells, which persist in confluent cultures for over 30 weeks. Similar to cultures derived from mouse marrow, corticosteroid-induced adipocyte differentiation is associated with long-term granulopoiesis. Human marrow preadipocytes, as well as human, mouse and rat embryo fibroblast cell lines, failed to differentiate to adipocyte morphology in the presence of insulin. In contrast, the 3T3-L1 insulin-dependent preadipocyte cell line was not induced to differentiate in the presence of hydrocortisone. These studies demonstrate that human marrow preadipocytes are dependent upon corticosteroid for differentiation in vitro.
Similar content being viewed by others
References
Greenberger, J. S. 1978. Sensitivity of corticosteroid-dependent, insulin resistant lipogenesis in marrow preadipocytes of mutation diabetic-obese mice. Nature 275: 752–754.
Bloom, W., and D. W. Fawcett. 1967. A Textbook of Histology, 8th Ed. Chapt. 5.Blood Cell Formation and Destruction. W. B. Saunders Co., Philadelphia, p. 120.
Wintrobe, M. M.Clinical Hematology, 6th Ed. Lea and Febinger Philadelphia, Chapt. 1, pp. 17–18. 1967.
Emery, J. L., and G. F. Follett. 1964. Regression of bone marrow hemopoiesis from the terminal digits in the fetus and infant. Br. J. Haematol. 10: 485–490.
Dexter, T. M., and N. G. Testa. 1976. Differentiation and proliferation of hemopoietic cells in culture. In: D. Prescott (Ed.),Methods in Cell Biology. Academic Press, New York, Vol. 14: 387–405.
Dexter, T. M., T. D. Allen, L. G. Lajtha, R. Schofield, and B. I. Lord. 1973. Stimulation of differentiation and proliferation of hemopoietic cells in vitro. J. Cell Physiol. 82: 461–470.
Greenberger, J. S., P. J. Gans, and P. B. Davisson. 1979. Murine sarcoma viruses block corticosteroid inducible differentiation of bone marrow preadipocytes associated with long-termin vitro hemopoiesis. Virology 95: 317–333.
Jainchill, J. C., S. A. Aaronson, and G. J. Todaro. 1969. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J. Virol. 4: 549–553.
Aaronson, S. A., and G. J. Todaro. 1968. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024–1026.
Duc-Nguyen, H., E. N. Rosenbaum, and R. F. Ziegel. 1966. Persistent infection of a rat kidney cell line with Rauscher leukemia virus. J. Bacteriol. 92: 113–1140.
Preece, A. 1972.Manual for Histologic Technicians. Little Brown and Co., Boston, p. 260.
Green, H., and O. Kehinde. 1974. Sublines of mouse 3T3 cells that accumulate lipid. Cell 1: 113–116.
Greenberger, J. S., A. Karpas, P. J. Gans, H. Newmann, and W. C. Moloney. 1978. Increased myeloid-associated enzymes in Epstein-Barr virus nuclear antigen—positive human cell lines exposed to butyric acidin vitro. Blood 51: 1073–1085.
Williams, N., H. Jackson, and E. M. Rabellino. 1977. Proliferation and differentiation of normal granulopoietic cells in continuous bone marrow cultures. J. Cell Physiol. 93: 435–441.
Greenberger, J. S., P. J. Gans, P. B. Davisson, and W. C. Moloney. 1979.In vitro induction of continuous acute promyelocytic leukemia cell lines by Friend or Abelson murine leukemia viruses. Blood 53: 987–1001.
Author information
Authors and Affiliations
Additional information
Supported by National Cancer Institute Virus Cancer Program Contract NCI NO1-7-1051.
Rights and permissions
About this article
Cite this article
Greenberger, J.S. Corticosteroid-dependent differentiation of human marrow preadipocytes in vitro. In Vitro 15, 823–828 (1979). https://doi.org/10.1007/BF02618309
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02618309