Skip to main content
SpringerLink
Log in

Isolation and characterization of viruses from fetal calf serum

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

Ten lots of specially procured fetal calf serum collected under sterile conditions and not filtered and 16 lots of commercial fetal calf serum were tested for both human and bovine viral contamination. The presence of viruses was evaluated by observing for cytopathogenic effect (CPE), hemadsorption with guinea pig erythrocytes, and interference with cytopathogenic challenge viruses in both embryonic bovine trachea (EBTr) and human diploid lung (HDL) cells.

Isolates were characterized by their cytopathogenicity, morphology, serology, and ability to propagate and produce cPE in a variety of bovine and nonbovine cells. One isolate was unequivocally identified as bovine herpes virus 1, and the other was presumptively identified as a bovine virus-diarrhea virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kniazeff, A. J., V. Rimer, and L. Gaeta. 1967. γ-Globulin in fetal bovine sera: significance in virology. Nature (Lond.) 214: 805–806.

    Article  CAS  Google Scholar 

  2. Malmquist, W. A. 1968. Bovine diarrhea-mucosal disease: etiology, pathogenesis and applied immunity. J. Am. Vet. Med. Assoc. 152: 763–768.

    Google Scholar 

  3. Schmidt, N. J. 1964. Tissue culture methods and procedures for diagnostic virology. In: C. H. Lennette and N. S. Schmidt (Eds.),Diagnostic Procedures for Virus & Rickettsial Diseases. Amer. Pub. Health Assoc., Inc., New York, pp. 78–176.

    Google Scholar 

  4. Kniazeff, A. J. 1968. Viruses infecting cattle and their role as endogenous contaminants of cell cultures. Natl. Cancer Inst. Monograph, Virus Vaccine Production. 29: 123–132.

    CAS  Google Scholar 

Download references

Author information

Author notes

  1. C. W. Molander

    Present address: City of Hope Medical Center, 91010, Duarte, Calif

Authors and Affiliations

  1. Hyland Division of Travenol Laboratories, University of California, San Diego

    C. W. Molander, A. J. Kniazeff, C. W. Boone, A. Paley & D. T. Imagawa

  2. National Cancer Institute, Bethesda, Maryland

    C. W. Molander, A. J. Kniazeff, C. W. Boone, A. Paley & D. T. Imagawa

  3. University of california, 90024, Los Angeles, California

    C. W. Molander, A. J. Kniazeff, C. W. Boone, A. Paley & D. T. Imagawa

Authors
  1. C. W. Molander
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. J. Kniazeff
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. W. Boone
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Paley
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. T. Imagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This study was supported by The National Institutes of Health under Contract PH 43-66-539.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Molander, C.W., Kniazeff, A.J., Boone, C.W. et al. Isolation and characterization of viruses from fetal calf serum. In Vitro 7, 168–173 (1971). https://doi.org/10.1007/BF02617962

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02617962

Keywords

Search

Navigation