Summary
A dye exclusion test for differentiating between live and dead tissue culture cells ofAedes aegypti is described. Erythrosin B at a final concentration of 20 mg/100 ml cell suspension stained these cells differentially; dead cells were stained red but the live ones did not take up the dye. There was a close correlation between the number of unstained cells and incorporation of14C-leucine. No significant increase was observed in the number of stained cells over a 1-hr staining period. Keeping the cells at 5°C up to 24 hr prior to addition of the dye affected neither total cell number nor the proportion of stained cells.
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References
Phillips, H. J., and J. E. Terryberry. 1957. Counting actively metabolizing tissue cultured cells. Exp. Cell. Res. 13: 341–347.
Hanks, J. H., and R. E. Wallace. 1958. Determination of cell viability. Proc. Soc. Exp. Biol. Med. 98: 188–192.
Kaltenbach, J. P., M. H. Kaltenbach, and W. B. Lyons. 1958. Nigrosin as a dye for differentiating live and dead ascites cells. Exp. Cell Res. 15: 112–117.
De Luca, C. 1965. The use of trypsin for the determination of cellular viability. Exp. Cell Res. 40: 186–188.
Paul, J., 1965.Cell and Tissue Culture. 3rd ed. E. & S. Livingstone, Ltd., Edinburgh.
Harris, M. 1966. Criteria of viability in heat-treated cells. Exp. Cell Res. 44: 658–661.
Grace, T. D. C. 1966. Establishment of a line of mosquito (Aedes aegypti L.) cells grownin vitro Nature (Lond.) 211: 366–367.
Grace, T. D. C. 1962. Establishment of four strains of cells from insect tissues grown in vitro. Nature (Lond.) 195: 788–789.
Sohi, S. S. 1969. Adaptation of anAedes aegypti cell line to hemolymph-free medium. Can. J. Microbiol. 15: 1197–1200.
Parker, R. C. 1961.Methods of Tissue Culture. 3rd ed. Paul B. Hoeber, Inc. New York.
Hayashi, Y., and S. S. Sohi, 1970. Metabolism ofAedes aegypti cells grown in vitro. 1. Incorporation of3H-uridine and14C-leucine. In Vitro 6: 148–152.
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Contribution 202, based on a paper read at the 21st Annual Meeting of the Tissue Culture Association held at Washington, D. C., June 15–18, 1970.
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Sohi, S.S., Hayashi, Y. Metabolism ofAedes aegypti cells grown in vitro. II. Determination of cell viability. In Vitro 7, 146–151 (1971). https://doi.org/10.1007/BF02617958
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DOI: https://doi.org/10.1007/BF02617958