Summary
A series of hydrolytic enzymes were compared with lysolecithin, glycerol monooleate, and inactivated Sendai virus for their ability to bring about the fusion of several human and mouse lymphoid cell lines. The agents were tried alone and in various combinations, and a variety of incubation conditions were tested to determine those optimal for fusion. Sendai virus was found to produce the best results with the mouse lymphoid cells; lysolecithin plus glycerol monooleate was slightly superior with the human lymphoid cells. A mixture of hyaluronidase plus collagenase produced low (2 to 6%), but significant, fusion of the human lymphoid cells; both the human and mouse lymphoid cell lines were found to contain relatively high amounts of prolyl hydroxylase, the enzyme which forms collagen from protocollagen. The maximum fusion obtained with the human cells was 16%; with a mouse plasmacytoma line, the maximum was 7.5%; and with a mouse leukemic line derived from L5178Y, the maximum was 60%.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Harris, H. 1970.Cell Fusion. Clarendon Press, Oxford.
Poste, A. 1970. Virus-induced polykaryocytosis and the mechanism of cell fusion. Adv. Virus Res. 16: 303–356.
Watkins, J. F. 1971. Fusion of cells for virus studies and production of cell hybrids. Methods Virol. 5: 1–33.
Stadler, J. K., and E. A. Adelberg. 1972. Cell cycle changes and the ability of cells to undergo virus-induced fusion. Proc. Natl. Acad. Sci. U.S.A. 69: 1929–1933. [See also correction: Stadler and Adelberg. 1973. Proc. Natl. Acad. Sci. U.S.A. 70: 965].
Poste, G. 1970. Virus-induced polykaryocytosis and the mechanism of cell fusion. Adv. Virus Res. 16: 303–356.
Poole, A. R., J. I. Howell, and J. A. Lucy. 1970. Lysolecithin and cell fusion. Nature 227: 810–813.
Croce, C. M., W. Sawicki, D. Kritchevsky, and H. Koprowski. 1971. Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithin. Exp. Cell Res. 67: 427–435.
Lucy, J. A., Q. F. Ahkong, F. C. Cramp, D. Fisher, and J. I. Howell. 1971. Cell fusion without viruses. Biochem. J. 124: 46P-47P.
Ahkong, Q. F., D. Fisher, W. Tampion, and J. A. Lucy. 1973. The fusion of erythrocytes by fatty acids, esters, retinol and α-tocopherol. Biochem. J. 136: 147–155.
Cramp, F. C., and J. A. Lucy. 1974. Glycerol monooleate as a fusogen for the formation of heterokaryons and interspecific hybrid cells. Exp. Cell Res. 87: 107–110.
Levy, J. A., M. Virolainen, and V. Defendi. 1968. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517–524.
Klebe, R. T., T.-R. Chen, and F. H. Ruddle. 1970. Controlled production of proliferating somatic cell hybrids. J. Cell Biol. 45: 74–82.
Langress, U., and S. Udenfriend. 1974. Collagen biosynthesis in non-fibroblastic cell lines. Proc. Natl. Acad. Sci. U.S.A. 71: 50–51.
Hutton, J. J., A. L. Tappel, and S. Udenfriend. 1966. A rapid assay for collagen proline hydroxylase. Anal. Biochem. 16: 384–394.
Author information
Authors and Affiliations
Additional information
This work was supported in part by NSF grant GB22864 (Yale) and Damon Runyon Memorial Fund for Cancer Research grant DRG-1239 (Iowa). This is Journal Paper No. J-7911 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa. Project No. 1986.
Rights and permissions
About this article
Cite this article
Stadler, J.K., Ward, A. & Adelberg, E.A. Optimal conditions for the fusion of lymphoid cell lines. In Vitro 11, 224–229 (1975). https://doi.org/10.1007/BF02616337
Issue Date:
DOI: https://doi.org/10.1007/BF02616337