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Characterization of the mouseTcra-V22 gene subfamily

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Abstract

T-cell receptors (Tcrs) of higher organisms play a key role in the specific recognition of self and non-self molecules in the immune system. The large number ofTcr variable (V) genes have been organized intoV gene subfamilies according to their sequence similarity at the nucleotide and amino acid level. We cloned and characterized four new members of theTcra-V22 gene subfamily at the genomic level using a simple and sensitive technique that can rapidly clone members of any multi-member gene family. Sequence analysis reveals that the fourTcra-V22 gene subfamily members have more than 98% sequence similarity in their coding regions, at the nucleotide and amino acid levels. However, the intron between the leader and the coding region varies up to 7% between members of theTcra-V22 gene subfamily. Comparison of the multimemberTcra-V22 gene subfamily with other multi-memberTcra-V gene subfamilies (V2, V8, andV11), shows thatTcra-V22 is unique in that it has multiple members with nearly identical amino acid sequence and which are not inherently pseudogenes. Sequence similarity analysis of theTcra-V22 subfamily with the prototypes of all otherTcra-V subfamilies revealed that theTcra-V22 subfamily has the closest sequence similarity to that ofTcra-V18 (77% at the nucleotide level and 71% at the amino acid level).

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Authors and Affiliations

  1. Center for Bioengineering, University of Washington, 98195, Seattle, WA, USA

    Anupam K. Dattamajumdar

  2. Department of Molecular Biotechnology, University of Washington School of Medicine, H-564 HSC, Box 357650, 98195, Seattle, WA, USA

    Sarah W. Li, David P. Jacobson, Leroy E. Hood & Gamal E. Osman

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  1. Anupam K. Dattamajumdar
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  2. Sarah W. Li
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  3. David P. Jacobson
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  4. Leroy E. Hood
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  5. Gamal E. Osman
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the EMBL/GenBank nucleotide sequence database and have been assigned the accession numbers U46076, U46077, U46078, U46079, and U47051

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Dattamajumdar, A.K., Li, S.W., Jacobson, D.P. et al. Characterization of the mouseTcra-V22 gene subfamily. Immunogenetics 44, 432–440 (1996). https://doi.org/10.1007/BF02602804

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  • DOI: https://doi.org/10.1007/BF02602804

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