Summary
Bone sialoprotein (BSP) and its messenger RNA were localized in developing human skeletal and nonskeletal tissues by means of immunohistochemistry andin situ hybridization. Both protein and mRNA were found in mature, bone-forming cells but not in their immature precursors. In addition, osteoclasts displayed positive immunostaining and high densities of autoradiographic grains byin situ hybridization experiments. BSP was expressed in fetal epiphyseal cartilage cells, particularly in hypertrophic chondrocytes of growth plates. Though neither the protein nor the mRNA were identified in a variety of other connective and nonconnective tissues, an unexpected finding was the expression of BSP in the trophoblast cells of placenta. These findings show that BSP is primarily an osteoblast-derived component of the bone matrix expressed at late stages of differentiation. We have also found that osteoclasts produce BSP, possibly as a mediator of cell attachment to bone.
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References
Herring GM (1972) The organic matrix of bone. In: Bourne GH (ed) The biochemistry and physiology of bone, vol 1. Academic Press, New York, pp 127–189
Fisher LW, Whitson SW, Avioli LV, Termine JD (1983) Matrix sialoprotein of developing bone. J Biol Chem 258:12723–12727
Fisher LW, Termine JD, Young MF (in press) Human bone sialoprotein: deduced protein sequence and chromosomal localization. J Biol Chem
Franzen A, Heinegard D (1985) Isolation and characterization of two sialoproteins present only in bone calcified matrix. Biochem J 232:715–724
Fisher L, Hawkins GR, Tuross N, Termine JD (1987) Purification and partial characterization of bone proteoglycans I and II, bone sialoproteins I and II and osteonectin from the mineral compartment of human bone. J Biol Chem 262:9702–9708
Kinne RW, Fisher LW (1987) Keratan sulfate proteoglycan in rabbit compact bone is bone sialoprotein II. J Biol Chem 262:10206–10211
Oldberg A, Franzen A, Heinegard D (1988) The primary structure of a cell-binding bone sialoprotein. J Biol Chem 263:19430–19432
Ruoslahti E, Pierschbacher MD (1987) New perspectives in cell adhesion: RGD and integrins. Science 238:491–497
Oldberg A, Franzen A, Heinegard D, Pierschbacher M, Ruoslahti E (1988) Identification of a bone sialoprotein receptor in osteosarcoma cells. J Biol Chem 263:19433–19436
Somerman MJ, Fisher LW, Foster RA, Sauk JJ (1988) Human bone sialoproteins I and II enhance fibroblast attachment in vitro. Calcif Tissue Int 43:50–53
Mintz KP, Midura RJ, Gehron Robey P, Termine JD, Fisher LW (1990) Attachment properties of non-denatured rat bone sialoprotein. J Bone Miner Res 5:S232
Ecarot-Charrier B, Bouchard S, Delloye C (1989) Bone sialoprotein II synthesized by cultured osteoblasts contains tyrosine sulfate. J Biol Chem 264:20049–20053
Midura RJ, McQuillan DJ, Benham KJ, Fisher LW, Hascall VC (1990) A rat osteogenic cell line (UMR 106-01) synthesizes a highly sulfated form of bone sialoprotein. J Biol Chem 265:5285–5291
Nomura S, Wills AJ, Edwards DR, Heath JK, Hoga BLM (1988) Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization. J Cell Biol 106:441–450
Graham RC, Karnovsky MJ (1966) The early stages of absorption of horseradish peroxidase in the proximal tubules of mouse kidneys: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–390
Cox KH, DeLeon DV, Angerer LM, Angerer RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol 101:485–502
Mark MP, Prince CW, Oosawa T, Gay S, Bronckers ALJJ, Butler WT (1987) Immunohistochemical demonstration of a 44-kD phosphoprotein in developing rat bones. J Histochem Cytochem 35:707–715
Mark MP, Butler WT, Prince CW, Finkelman RD, Ruch JV (1988) Developmental expression of 44-kD bone phosphoprotein (osteopontin) and bone-carboxyglutamic acid (Gla) containing protein (osteocalcin) in calcifying tissues of rat. Differentiation 37:123–136
Bianco P, Silverstrini G, Termine JD, Bonucci E (1988) Immunohistochemical localization of osteonectin in human and calf developing bone using monoclonal antibodies. Calcif Tissue Int 43:155–161
Bianco P, Fisher LW, Young MF, Kopp JB, Termine JD, Gehron Robey P (1990) The use of synthetic peptide antibodies and in situ hybridization for investigating expression and localization of small proteoglycans of developing bone (biglycan and decorin). In: Cohn DV, Glorieux FH, Martin TJ (eds) Proceedings ASBMR/ICCRH. Elsevier, Amsterdam, pp 201–206
Baron R, Neff L, Louvard D, Courtoy JP (1985) Cell-mediated extracellular acidification and bone resorption: evidence for a low pH in resorbing lacunae and localization of a 100-kD lysosomal membrane protein at the osteoclast ruffled border. J Cell Biol 101:2210–2222
Metsaranta M, Young MF, Sandberg M, Termine JD, Vuorio E (1989) Localization of osteonectin expression in human fetal skeletal tissues by in situ hybridization. Calcif Tissue Int 45:146–152
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Bianco, P., Fisher, L.W., Young, M.F. et al. Expression of bone sialoprotein (BSP) in developing human tissues. Calcif Tissue Int 49, 421–426 (1991). https://doi.org/10.1007/BF02555854
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DOI: https://doi.org/10.1007/BF02555854