Protoplasma

, Volume 214, Issue 1, pp 65–72

Phloem loading in the sucrose-export-defective (SXD-1) mutant maize is limited by callose deposition at plasmodesmata in bundle sheath—vascular parenchyma interface

  • C. E. J. Botha
  • R. H. M. Cross
  • A. J. E. van Bel
  • C. I. Peter
Original Articles

DOI: 10.1007/BF02524263

Cite this article as:
Botha, C.E.J., Cross, R.H.M., van Bel, A.J.E. et al. Protoplasma (2000) 214: 65. doi:10.1007/BF02524263

Summary

Using Lucifer Yellow we have demonstrated that the phloem-loading pathway from the mesophyll to the bundle sheath—vascular parenchyma interface inZea mays source leaves follows a symplasmic route in small and intermediate vascular bundles in control as well as in the green sections of mutant sucrose-export-defective (SXD-1) plants. In the anthocyanin-rich mutant leaf sections, Lucifer Yellow transport was prohibited along the same path, at the bundle sheath—vascular parenchyma interface in particular. Plasmodesmata at the latter interface in SXD-1 anthocyanin-rich leaf sections appear to be structurally altered through callose deposition at the plasmodesmal orifices. We suggest that a transport bottleneck at the bundle sheath—vascular parenchyma interface is thus orchestrated and regulated through callose formation, preventing symplasmic transport across this important loading interface.

Keywords

CalloseLucifer yellowPlasmodesmaPhloem loadingSucrose-export-defective mutantZea mays

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • C. E. J. Botha
    • 1
  • R. H. M. Cross
    • 2
  • A. J. E. van Bel
    • 3
  • C. I. Peter
    • 1
  1. 1.Botany DepartmentRhodes UniversityGrahamstownSouth Africa
  2. 2.Electron Microscopy UnitRhodes UniversityGrahamstown
  3. 3.Institut für Allgemeine Botanik und PflanzenphysiologieJustus-Liebig Universität GiessenGiessen