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Phloem loading in the sucrose-export-defective (SXD-1) mutant maize is limited by callose deposition at plasmodesmata in bundle sheath—vascular parenchyma interface

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Summary

Using Lucifer Yellow we have demonstrated that the phloem-loading pathway from the mesophyll to the bundle sheath—vascular parenchyma interface inZea mays source leaves follows a symplasmic route in small and intermediate vascular bundles in control as well as in the green sections of mutant sucrose-export-defective (SXD-1) plants. In the anthocyanin-rich mutant leaf sections, Lucifer Yellow transport was prohibited along the same path, at the bundle sheath—vascular parenchyma interface in particular. Plasmodesmata at the latter interface in SXD-1 anthocyanin-rich leaf sections appear to be structurally altered through callose deposition at the plasmodesmal orifices. We suggest that a transport bottleneck at the bundle sheath—vascular parenchyma interface is thus orchestrated and regulated through callose formation, preventing symplasmic transport across this important loading interface.

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References

  • Allison JCS, Weinmann H (1970) Effect of absence of developing grain on carbohydrate content and senescence of maize leaves. Plant Physiol 46: 435–436

    PubMed  CAS  Google Scholar 

  • Apitius A, Lehmann H (1995) The deposition of different wall materials as a wound reaction in the liverwortRiella helicophylla. Cryptogam Bot 5: 351–359

    Google Scholar 

  • Beebe DU, Turgeon R (1991) Current perspectives on plasmodesmata structure and function. Physiol Plant 83: 194–199

    Article  CAS  Google Scholar 

  • Blackman LM, Gunning BES, Overall RL (1998) A 45 kDa protein isolated from the nodal walls ofChara corallina is localised to plasmodesmata. Plant J 15: 401–411

    Article  CAS  Google Scholar 

  • Botha CEJ (1988) Plasmodesmatal distribution and frequency in vascular bundles and contiguous tissue of the leaf ofThemeda triandra. Planta 173: 433–441

    Article  Google Scholar 

  • —, Cross RHM (1997) Plasmodesmatal frequency in relation to short-distance transport and phloem loading in the leaves of barley (Hordeum vulgare): phloem is not loaded directly from the symplast. Physiol Plant 99: 355–362

    Article  CAS  Google Scholar 

  • Ding B (1998) Intercellular protein trafficking through plasmodesmata. Plant Mol Biol 38: 279–310

    Article  PubMed  CAS  Google Scholar 

  • —, Haudenshield JS, Hull RJ, Wolf S, Beachy RN, Lucas WJ (1992) Secondary plasmodesmata are specific sites of localization of the tobacco mosaic movement protein in transgenic tobacco plants. Plant Cell 4: 915–928

    Article  PubMed  CAS  Google Scholar 

  • — —, Willmitzer L, Lucas WJ (1993) Correlation between arrested secondary plasmodesmal development and the onset of accelerated leaf senescence in yeast acid invertase transgenic plants. Plant J 4: 179–189.

    Article  PubMed  CAS  Google Scholar 

  • Erwee MG, Goodwin PB (1983) Characterisation of theEgeria densa Planch, leaf symplast: inhibition of the intercellular movement of fluorescent probes by group II ions. Planta 158: 320–328

    Article  CAS  Google Scholar 

  • Evert RF, Eschrich W, Heyser W (1977) Distribution and structure of plasmodesmata in mesophyll and bundle-sheath cells ofZea mays. Planta 136: 77–89

    Article  Google Scholar 

  • — — — (1978) Leaf structure in relation to solute transport and phloem loading inZea mays L. Planta 138: 279–294

    Article  Google Scholar 

  • —, Russin WA, Botha CEJ (1996) Distribution and frequency of plasmodesmata in relation to photoassimilate pathways and phloem loading in the barley leaf. Planta 198: 572–579

    Article  CAS  Google Scholar 

  • Gamalei YV (1989) Structure and function of leaf minor veins in trees and herbs: a taxonomic review. Trees 3: 96–110

    Article  Google Scholar 

  • — (1991) Phloem loading and its development related to plant evolution from trees to herbs. Trees 5: 50–64

    Article  Google Scholar 

  • Goodrick BJ, Kuhn CW, Hussey RS (1991) Restricted systemic movement of cowpea chlorotic mottle virus in soybean with non-necrotic resistance. Phytopathology 81: 1426–1431

    Google Scholar 

  • Hughes JE, Gunning BES (1980) Glutaraldehyde-induced deposition of callose. Can J Bot 58: 250–258

    CAS  Google Scholar 

  • Kalt-Torres W, Kerr PS, Usuda H, Huber SC (1987) Diurnal changes in maize leaf photosynthesis. Plant Physiol 83: 283–288

    PubMed  CAS  Google Scholar 

  • Kempers R, van Bel AJE (1997) Symplasmic connections between sieve element and companion cell in the stem phloem ofVicia faba L have a molecular exclusion limit of at least 10 kDa. Planta 201: 195–200

    Article  CAS  Google Scholar 

  • —, Prior DAM, Oparka KJ, Knoblauch M, van Bel AJE (1999) Integration of controlled intercellular pressure injection, iontophoresis and membrane potential measurement. Plant Biol 1: 61–67

    Google Scholar 

  • Kollmann R, Glockmann C (1999) Multimorphology and nomenclature of plasmodesmata in higher plants. In: van Bel AJE, van Kesteren WJP (eds) Plasmodesmata: structure, function, role in cell communication. Springer, Berlin Heidelberg New York Tokyo, pp 149–172

    Google Scholar 

  • Lucas WJ, Olesinski A, Hull RJ, Haudenshield JS, Deom CM, Wolf S, Beachy RN (1993) Influence of the tobacco mosaic virus 30-kDa movement protein on carbon metabolism and photosynthate partitioning in transgenic tobacco plants. Planta 190: 88–96

    Article  CAS  Google Scholar 

  • Nelson T, Dengler N (1997) Leaf vascular pattern formation. Plant Cell 9: 1121–1135

    Article  PubMed  CAS  Google Scholar 

  • Radford JE, Vesk M, Overall RL (1998) Callose deposition at plasmodesmata. Protoplasma 201: 30–37

    Article  CAS  Google Scholar 

  • Rhoades MM, Carvalho A (1944) The function and structure of the parenchyma sheath plastids of the maize leaf. Bull Torrey Bot Club 71: 335–346

    Article  Google Scholar 

  • Robards AW, Lucas WJ (1990) Plasmodesmata. Annu Rev Plant Physiol Plant Mol Biol 41: 369–419

    Article  Google Scholar 

  • Russin WA, Evert RF, Vanderveer PJ, Sharkey TD, Briggs SP (1996) Modification of a specific class of plasmodesmata and loss of sucrose export ability in the sucrose export defectivel maize mutant. Plant Cell 8: 645–658

    Article  PubMed  CAS  Google Scholar 

  • Schulz A (1999) Physiological control of plasmodesmal gating. In: van Bel AJE, van Kesteren WJP (eds) Plasmodesmata: structure, function, role in cell communication. Springer, Berlin Heidelberg New York Tokyo, pp 173–204

    Google Scholar 

  • Thompson JR, Garcia-Arenal F (1998) The bundle-sheath phloem interface ofCucumis sativus is a boundary to systemic infection by tomato aspermy virus. Mol Plant Microbe Interact 11: 109–114

    CAS  Google Scholar 

  • Tucker EB (1990) Calcium-loaded 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetra acetic acid blocks cell-to-cell diffusion of carboxyfluorescein in staminal hairs ofSetcreasea purpurea. Protoplasma 174: 45–49

    Article  Google Scholar 

  • —, Boss WF (1996) Mastoparan-induced intracellular Ca2+ fluxes may regulate cell-to-cell communication in plants. Plant Physiol 111: 459–467

    PubMed  CAS  Google Scholar 

  • Turgeon R, Beebe DU (1991) The evidence for symplasmic phloem loading. Plant Physiol 96: 349–354

    Article  PubMed  CAS  Google Scholar 

  • van Bel AJE (1992) Different phloem-loading machineries correlated with the climate. Acta Bot Neerl 41: 121–141

    Google Scholar 

  • — (1993) Strategies of phloem loading. Annu Rev Plant Physiol Plant Mol Biol 44: 253–281

    Article  Google Scholar 

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Authors and Affiliations

  1. Botany Department, Rhodes University, Lucas Avenue, 6140, Grahamstown, South Africa

    C. E. J. Botha & C. I. Peter

  2. Electron Microscopy Unit, Rhodes University, Grahamstown

    R. H. M. Cross

  3. Institut für Allgemeine Botanik und Pflanzenphysiologie, Justus-Liebig Universität Giessen, Giessen

    A. J. E. van Bel

Authors
  1. C. E. J. Botha
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  2. R. H. M. Cross
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  3. A. J. E. van Bel
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  4. C. I. Peter
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Correspondence to C. E. J. Botha.

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Botha, C.E.J., Cross, R.H.M., van Bel, A.J.E. et al. Phloem loading in the sucrose-export-defective (SXD-1) mutant maize is limited by callose deposition at plasmodesmata in bundle sheath—vascular parenchyma interface. Protoplasma 214, 65–72 (2000). https://doi.org/10.1007/BF02524263

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  • DOI: https://doi.org/10.1007/BF02524263

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