Abstract
A method for obtaining delay histograms for the time course of neurotransmitter release is presented. The delay histogram is derived from the measured psc (or the sum of several psc's) and the mpsc (obtained experimentally or otherwise) by means of a simple, quick, mathematical procedure. The procedure may be automated for the greater part. No approximation of the mpsc shape is performed, and the method is applicable to all quantal contents.
For low and medium quantal contents, the delay histograms obtained by the method are compared to those obtained by direct analysis. A reasonable agreement is achieved. An experiment of high quantal content, for which direct analysis is impossible, is then analysed using the new method.
Difficulties which may arise when applying the procedure and methods to overcome them are discussed at length. Other methods are set forth in the Discussion.
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Literature
Barrett, E. F. and C. F. Stevens. 1972. The kinetics of transmitter release at the frog neuromuscular junction.J. Physiol. (Lond.) 227, 691–708.
Cohen, I. S. W., W. Van der Kloot and D. Attwell. 1981. The timing of channel opening during minature end-place currents.Brain Res. 223, 185–189.
Dempster, J. 1986. The use of driving function in the analysis of endplate current kinetics.J. Neurosci. Methods 18, 277–285.
Dudel, J. 1981. The effect of reduced calcium on quantal unit current at the crayfish neuromuscular junction.Pflugers Arch. 391, 35–40.
Glusman, S. and E. A. Kravitz. 1982. The action of seratonin of excitatory nerve terminals in lobster nerve-muscle preparations.J. Physiol. (Lond.) 325, 223–241.
Katz, B. and R. Miledi. 1965. The measurement of synaptic delay, and the time course of acetylcholine release at the neuromuscular junction.Proc. R. Soc. B 161, 483–493.
Lustig, C., H. Parnas and L. Segel. 1990. Release kinetics as a tool to describe drug effects on neurotransmitter release.J. theor. Biol., in press.
Neher, E., B. Sakman and J. H. Steinbach. 1978. The extracellular patch clamp: A method for resolving currents through individual open channels in biological membranes.Pflugers Arch.375, 219–228.
Parnas, H., I. Parnas and L. Segel. 1986. A new method for determining co-operativity in neurotransmitter release.J. theor. Biol. 119, 481–499.
Parnas, H., M. Flashner and M. E. Spira. 1989. Sequential model to describe the nicotinic synaptic current.Biophys. J. 55, 875–884.
Van der Kloot, W. 1988. Estimating the timing of quantal releases during end-plate currents at the frog neuromuscular junction.J. Physiol. (Lond.) 402, 595–603.
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Aumann, Y., Parnas, H. Evaluation of the time course of neurotransmitter release from the measured PSC and MPSC. Bltn Mathcal Biology 53, 537–555 (1991). https://doi.org/10.1007/BF02458628
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DOI: https://doi.org/10.1007/BF02458628