Abstract
The four most common allozymes at thePgm-2 locus inCrassostrea gigas were purified and characterized over physiological ranges of temperature andpH. Significant differences were observed between genotypes in their apparent Michaelis constants for glucose-1-phosphate and glucose-1,6-diphosphate,V max/K m ratios,pH-dependent activities, and temperature stabilities. These functional differences were caused almost exclusively by the divergent properties of thePgm-292 allozyme; limited differentiation existed among thePgm-296,Pgm-2100, andPgm-2104 variants. Heterozygotes displayed strict intermediacy for all kinetic and structural properties examined. The results are discussed in light of their ability to account for the overdominant body weights ofPgm-2 heterozygotes reported by Fujio (1982). It is concluded that overdominance is unlikely to arise at this locus as a consequence of these biochemical differences because of their limited magnitude and incompatibility with allelic frequencies in natural populations.
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Funding for the study was furnished by NSERC operating grants to C. F. Wehrhahn.
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Pogson, G.H. Biochemical characterization of genotypes at the phosphoglucomutase-2 locus in the pacific oyster,Crassostrea gigas . Biochem Genet 27, 571–589 (1989). https://doi.org/10.1007/BF02396153
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DOI: https://doi.org/10.1007/BF02396153