Abstract
By treatment of chorioallantoic membranes from embryonated eggs with collagenase and hyaluronidase before the conventional application of trypsin cells could be grown in culture which supported growth of a large variety of myxoviruses, herpesviruses, avian reoviruses and the infectious bronchitis virus of chickens. The cultures could be used for sensitive plaque assays and neutralization tests.
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This work was supported by the Sonderforschungsbereich 47 (Virologie).
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Cursiefen, D., Becht, H. In vitro cultivation of cells from the chorioallantoic membrane of chick embryos. Med Microbiol Immunol 161, 3–10 (1975). https://doi.org/10.1007/BF02120764
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DOI: https://doi.org/10.1007/BF02120764