Summary
A method is described for the complete dissociation of mouse hair follicles into a suspension of single cells suitable for cell culture. Dermal tissue containing hair follicles is digested sequentially with trypsin and chondroitinase ABC under mild conditions.
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References
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Frater, R. A new technique for dissociation of hair follicles into single cells. Experientia 32, 675–676 (1976). https://doi.org/10.1007/BF01990230
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DOI: https://doi.org/10.1007/BF01990230