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Histopathological method for diagnosis of cryptosporidiosis using monoclonal antibodies

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Abstract

A monoclonal antibody directed against the oocyst wall ofCryptosporidium parvum was produced which strongly reacted with 25 strains of the parasite. This antibody was assessed for use in a diagnostic immunoperoxidase assay performed on tissue sections. It permitted an intense and specific staining of the parasite in five biopsies positive forCryptosporidium parvum fixed with routine techniques. The procedure described may be useful in screening mucosal biopsies from immunosuppressed patients.

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Authors and Affiliations

  1. Laboratoire de Parasitologie-Mycologie, Hôpital du Bocage, 2 Bd. Maréchal-de-Lattre-de-Tassigny, 21034, Dijon Cedex, France

    A. Bonnin & P. Camerlynck

  2. Laboratoire d'Anatomie Pathologique, Faculté de Médecine, 21033, Dijon Cedex, France

    T. Petrella

  3. INSERM U 42, Domaine du Certia, 59650, Villeneuve d'Ascq, France

    J. F. Dubremetz

  4. Laboratoire d'Anatomie Pathologique, Hôpital Pasteur, 06000, Nice, France

    J. F. Michiels

  5. Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, 51092, Reims Cedex, France

    D. Puygauthier-Toubas

Authors
  1. A. Bonnin
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  2. T. Petrella
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  3. J. F. Dubremetz
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  4. J. F. Michiels
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  5. D. Puygauthier-Toubas
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  6. P. Camerlynck
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Bonnin, A., Petrella, T., Dubremetz, J.F. et al. Histopathological method for diagnosis of cryptosporidiosis using monoclonal antibodies. Eur. J. Clin. Microbiol. Infect. Dis. 9, 664–666 (1990). https://doi.org/10.1007/BF01964268

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  • DOI: https://doi.org/10.1007/BF01964268

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