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Ouabain-resistant Na+, K+ transport system in mouse NIH 3T3 cells

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Summary

It is shown that the ouabain-resistant (OR) furosemide-sensitive K+(Rb+) transport system performs a net efflux of K+ in growing mouse 3T3 cells. This conclusion is based on the finding that under the same assay conditions the furosemidesensitive K+(Rb+) efflux was found to be two- to threefold higher than the ouabain-resistant furosemide-sensitive K+(Rb+) influx. The oubain-resistant furosemide-sensitive influxes of both22Na and86Rb appear to be Cl− dependent, and the data are consistent with coupled unidirectional furosemide-sensitive influxes of Na+, K+ and Cl− with a ratio of 1 ∶ 1 ∶ 2. However, the net efflux of K+ performed by this transport system cannot be coupled to a ouabain-resistant net efflux of Na+ since the unidirectional ouabain-resistant efflux of Na+ was found to be negligible under physiological conditions. This latter conclusion was based on the fact that practically all the Na+ efflux appears to be ouabainsensitive and sufficient to balance the Na+ influx under such steady-state conditions. Therefore, it is suggested that the ouabain-resistant furosemide-sensitive transport system in growing cells performs a facilitated diffusion of K+ and Na+, driven by their respective concentration gradients: a net K+ efflux and a net Na+ influx.

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Authors and Affiliations

  1. Department of Medical Biophysics, Hadassah University Hospital, Jerusalem, Israel

    Henri Atlan, David Snyder & Rivka Panet

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  1. Henri Atlan
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  2. David Snyder
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  3. Rivka Panet
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Atlan, H., Snyder, D. & Panet, R. Ouabain-resistant Na+, K+ transport system in mouse NIH 3T3 cells. J. Membrain Biol. 81, 181–188 (1984). https://doi.org/10.1007/BF01868712

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  • DOI: https://doi.org/10.1007/BF01868712

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