Summary
Goblet cell mucin in 39 human colons was studied by methods specific for various sugar residues, including staining with three lectins,Dolichos biflorus agglutinin (DBA, specific for blood group A antigen),Griffonia simplicifolia agglutinin-I (GSA-I, B) and peanut agglutinin (PNA, T antigen), and immunostaining for A, B, H and T. Isoantigens A, B or H were found only in the right colon. GSA-I reactive goblet cells occurred in the right colon of both blood group A and B patients and possibly contained isoantigens. However DBA reactive cells were found in all cases. Prior neuraminidase digestion imparted anti-A, GSA-I and DBA reactivities to the cells lining the lower crypts in all cases. This pretreatment also imparted PNA and anti-T reactivities to goblet cells, only the latter reactivity being eliminated by galactose oxidase. Goblet cell mucin in transitional mucosa revealed decreased A and B, and increased H antigens. Enhanced galactose oxidase—Schiff (GOS) and anti-T reactivities were also noted. The present results revealed that some lectin reactions of goblet cells might be related to blood group antigens but others were not, and that different techniques for demonstrating reputedly the same sugar residues produced different results, indicating a need for proper evaluation of their specificity.
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ALLEN, A. K., NEUBERGER, A. & SHARON, N. (1973) The purification, composition and specificity of wheatgerm agglutinin.Biochem. J. 131, 155–62.
AVIGAD, G., AMARAL, D., ASENSIO, C. & HORECKER, B. L. (1962) The D-galactose oxidase of polyporus circinatus.J. Biol. Chem. 237, 2736–43.
BIRD, G. W. G (1952) Relationship of the blood sub-groups A1, A2 and A1B, A2B to haemagglutinins present in the seeds ofDolichos biflorus.Nature 170, 674.
BIRD, G. W. G. (1964) Anti-T in peanuts.Vox Sang. 9, 748–9.
BOLAND, C. R., MONTGOMERY, C. K. & KIM, Y. S. (1982) Alterations in human colonic mucin occurring with cellular differentiation and malignant transformation.Proc. Natl. Acad. Sci. USA. 79, 2051–5.
BRESALIER, R. S., BOLAND, C. R. & KIM, Y. S. (1985) Regional differences in normal and cancer-associated glycoconjugates of the human colon.J. Natl. Cancer Inst. 75, 249–60.
COOPER, H. S. (1982) Peanut lectin-binding sites in large bowel carcinoma.Lab. Invest. 47, 383–90.
CULLING, C. F. A. & REID, P. E. (1979) The histochemistry of colonic mucins.J. Histochem. Cytochem. 27, 1177–9.
CULLING, C. F. A., REID, P. E. & DUNN, W. L. (1976) A new histochemical method for the identification and visualization of both side chain acylated and nonacylated sialic acids.J. Histochem. Cytochem. 24, 1225–30.
ETZLER, M. E. & KABAT, E. A. (1970) Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity fromDolichos biflorus.Biochemistry 9, 869–77.
FEIZI, T., COOI, H. C., CHILDS, R. A., PICARD, J. K., UEMURA, K., LOOMES, L. M., THORPE, S. J. & HOUNSELL, E. F. (1984) Tumor-associated and differentiation antigens on the carbohydrate moieties of mucin-type glycoproteins.Biochem. Soc. Trans. 12, 591–6.
FILIPE, M. I. & BRANFOOT, A. C. (1974) Abnormal patterns of mucous secretion in apparently normal mucosa of large intestine with carcinoma.Cancer 34, 282–90.
GATHMANN, W. D. & AMINOFF, D. (1981) Steric factors involved in the action of glycosidases and galactose oxidase.Biochem. Biophys. Res. Commun. 103, 68–76.
GOLDSTEIN, I. J. & HAYES, C. E. (1978) The lectins: carbohydrate-binding proteins of plants and animals. InAdvances in Carbohydrate Chemistry and Biochemistry (edited by TIPSON, R. S. and HORTON, D.), pp. 127–340. New York: Academic Press.
GUESDON, J. L., TERNYNCK, T. & AVRAMEAS, S. (1979) The use of avidin—biotin interaction in immunoenzymatic techniques.J. Histochem. Cytochem. 27, 1131–9.
HAYES, C. E. & GOLDSTEIN, I. J. (1974) An α-d-galactosylbinding lectin fromBandeiraea simplicifolia seeds.J. Biol. Chem. 249, 1904–14.
HOTTA, K., GOSO, K. & KATO, Y. (1982) Human gastric glycoproteins corresponding to paradoxical concanavalin A staining.Histochemistry 76, 107–12.
ISAACSON, P. & ATTWOOD, P. R. A. (1979) Failure to demonstrate specificity of the morphological and histochemical changes in mucosa adjacent to colonic carcinoma (transitional mucosa).J. Clin. Pathol. 32, 214–8.
KATSUYAMA, T., ONO, K., NAKAYAMA, J., AKAMATSU, T. & HONDA, T. (1985a) Mucosubstance histochemistry of the normal mucosa and carcinoma of the large intestine. Galactose oxidase—Schiff reaction and lectin stainings.Acta Pathol. Jpn 35, 1409–25.
KATSUYAMA, T., ONO, K., NAKAYAMA, J., & KANAI, M. (1985b) Recent advances in mucosubstance histochemistry, InGastric Mucous and Mucous Secreting Cells (edited by KAWAI, K.), pp. 3–18. Amsterdam: Excerpta Medica.
KATSUYAMA, T. & SPICER, S. S. (1978) Histochemical differentiation of complex carbohydrates with variants of the concanavalin A-horseradish peroxidase method.J. Histochem. Cytochem. 26, 233–50.
LISOWSKA, E. & KORDIWICZ, M. (1977) Immunochemical properties of M and N blood group antigens and their degradation products. InHuman Blood Groups, Proceedings of the 5th International Convocation on Immunology (edited by MOHN, J. F., PLUNKETT, R. W., CUNNINGHAM, R. K. and LAMBERT, R. M.), pp. 188–96. Basel: S. Karger AG.
LOTAN, R., SKUTELSKY, E., DANON, D. & SHARON, N. (1975) The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea).J. Biol. Chem. 250, 8513–23.
MURPHY, L. A. & GOLDSTEIN, I. J. (1977) Five α-d-galactopyranosyl-binding isolectins fromBandeiraea simplicifolia Seeds.J. Biol. Chem. 252, 4739–42.
NAKAMURA, T., YONEZAWA, S., TANAKA, S. & SATO, E. (1985) Lectin histochemistry in colorectal adenomas and early carcinomas.Pathol. Clin. Med. 3, 317–22 (in Japanese).
NAKAYAMA, J., KATSUYAMA, T., ONO, K., HONDA, T., AKAMATSU, T. & HATTORI, H. (1985) Large bowel carcinoma-specific antigens detected by the lectin,Griffonia simplicifolia agglutinin-II.Jpn J. Cancer Res. (Gann)76, 1078–84.
ONO, K. & KATSUYAMA, T. (1985) Mucosubstance histochemistry of the normal mucosa and epithelial neoplasms of the large intestine.Acta. Pathol. Jpn 35, 281–93.
RACE, R. R. & SANGER, R. (1975)Blood Groups in Man, 6th edn. Oxford: Blackwell Scientific.
REID, P. E., CULLING, C. F. A., DUNN, W. L., RAMEY, C. W. & CLAY, M. G. (1984) Chemical and biochemical studies of normal and diseased human gastrointestinal tract. I. A comparison between histologically normal colon, colonic tumours, ulcerative colitis and diverticular disease of the colon.Histochem. J. 16, 235–51.
SATO, A. & SPICER, S. S. (1982) Ultrastructural visualization of galactose in the glycoprotein of gastric surface cells with a peanut lectin conjugate.Histochem. J. 14, 125–38.
SCHULTE, B. A. & SPICER, S. S. (1983) Light microscopic histochemical detection of terminal galactose andN-acetylgalactosamine residues in rodent complex carbohydrates using a galactose oxidase—Schiff sequence and peanut lectin-horseradish peroxidase conjugate.J. Histochem. Cytochem. 31, 19–24.
SHANKAR-IYER, P. N., WILKINSON, K. D. & GOLDSTEIN, I. J. (1976) AnN-acetyl-d-glucosamine binding lectin fromBandeiraea simplicifolia seeds.Arch. Biochem. Biophys. 177, 330–3.
SPICER, S. S. (1965) Diamine methods for differentiating mucopolysaccharides histochemistry.J. Histochem. Cytochem. 13, 211–34.
WILEY, E. L., MURPHY, P., MENDELSOHN, G. & EGGLESTON, J. C. (1981) Distribution of blood group substances in normal human colon. Use of the unlabeled antibody (PAP) immunoperoxidase technic to identify A and B blood group substances.Amer. J. Clin. Pathol. 76, 806–9.
YAMADA, K. & SHIMIZU, S. (1977) The histochemistry of galactose residues of complex carbohydrates as studied by peroxidase-labeledRicinus communis agglutinin.Histochemistry 53, 143–56.
YONEZAWA, S., NAKAMURA, T., TANAKA, S. & SATO, E. (1982) Glycoconjugate withUlex europeus agglutinin-1-binding sites, in normal mucosa, adenoma and carcinoma of the human large bowel.J. Natl. Cancer Inst. 69, 777–85.
YUAN, M., ITZKOWITZ, S. H., PALEKAR, A., SHAMSUDDIN, A. M., PHELPS, P. C., TRUMP, B. F. & KIM, Y. S. (1985) Distribution of blood group antigens A, B, H, Lewisa, and Lewisb in human normal, fetal, and malignant colonic tissue.Cancer Res. 45, 4499–511.
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Nakayama, J., Ota, M., Honda, T. et al. Histochemical demonstration of sugar residues by lectin and immunocytochemical techniques for blood group antigens in human colon. Histochem J 19, 454–464 (1987). https://doi.org/10.1007/BF01675757
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DOI: https://doi.org/10.1007/BF01675757