Summary
This investigation has shown the following hierarchy of chloroplast reactions which were susceptible to increasing glutaraldehyde concentration given for 6 min at 0° C.
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(1)
Cyclic and non-cyclic photophosphorylation was completely inhibited by 100 mM glutaraldehyde.
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(2)
Reduction in the rate of H+ uptake, and increase in the rate of H+ efflux occurred to give a ratio uptake/loss of unity with 100 mM glutaraldehyde.
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(3)
Volume fixation occurred at 200 mM glutaraldehyde.
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(4)
Fixed chloroplasts were resistant to stimulation of H+ and electron transport by weak acid anions and uncouplers.
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(5)
Light-induced electron-transport activity progressively decreased. About 35% inhibition of the basal (non-phosphorylating) rate and 60% inhibition of the uncoupled rate occurred on fixation.
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(6)
The extent of the light-induced H+ changes were reduced only by about 10% upon fixation.
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West, J., Packer, L. The effect of glutaraldehyde on light-induced H+ changes, electron transport, and phosphorylation in pea chloroplasts. J Bioenerg Biomembr 1, 405–412 (1970). https://doi.org/10.1007/BF01654577
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DOI: https://doi.org/10.1007/BF01654577