Abstract
Five embryonic mouse cultures and one human fibroblast culture were transformed with SV40. The cultures were studied cytologically to see if the normal pattern of sex chromosome replication was maintained in SV40 transformed cells. Characteristic late replication patterns were observed for both the X and Y chromosomes, and there was no evidence for loss of the inactive X chromosome, even in cells with 4 or more X chromosomes. The human line was heterozygous at two X-linked loci and a clonal analysis showed that the expression of X-linked genes was not affected by SV40 transformation.
Similar content being viewed by others
Literature cited
Lyon, M. F. (1962).Am. J. Hum. Genet. 14:135–148.
Davidson, R. G., Nitowsky, H. M., and Childs, B. (1963).Proc. Natl. Acad. Sci. U.S.A. 50:481–485.
Gartler, S. M., and Andina, R. J. (1976).Adv. Hum. Genet. 7:99–140.
Cattanach, B. M. (1974).Genet. Res. Cambridge 23:291–306.
Kahan, B., and DeMars, R. (1975).Proc. Natl. Acad. Sci. U.S.A. 72:1510–1514.
Johnston, P. G., Sharman, G. B., James, E. A., and Cooper, D. W. (1978).Aust. J. Biol. Sci. 31:415–424.
Black, P. H. and Rowe, W. P. (1963).Proc. Soc. Exp. Biol. Med. 114:721–727.
Bartholomew, E. M., Bartholomew, W. R., and Rose, W. R. (1969).J. Immunol. 103:787–794.
Nelson-Rees, W. A., and Scher, C. D. (1973).Bibl. Haematol. Basel 39:524–535.
Oppenheim, A., and Martin, R. G. (1978).J. Virol. 25:450–452.
Romeo, G., and Migeon, B. R. (1975).Humangenetik 29:165–170.
Zuna, R. G., and Lehman, J. M. (1977).J. Natl. Cancer Inst. 58:1463–1472.
Raskind, W. H., and Gartler, S. M. (1978).Somat. Cell Genet. 4:491–506.
Gartler, S. M., Chen, S., Fialkow, P. J., and Giblett, E. R. (1972).Nature 236:149–150.
Farber, R. A. (1973).Genetics 74:521–531.
Callis, J., and Hoehn, H. (1976).Am. J. Hum. Genet. 28:577–584.
Smith, H. S., Scher, C. D., and Todaro, G. J. (1971).Virology 44:359–370.
Smith, H. S., Gelb, L. D., and Martin, M. A. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:152–156.
Kraemer, P. M., Petersen, D. F., and Van Dilla, M. A. (1971).Science 174:714–717.
Tavares, A. S. (1966). InThe Sex Chromatin, (ed.) Moore, K. L. (Saunders, Philadelphia), pp. 405–433.
Nesbitt, M. N., and Gartler, S. M. (1970).Cytogenet. 9:212–221.
Farber, R. A., and Liskay, R. M. (1974).Cytogenet. Cell Genet. 13:384–396.
Rosenstraus, M. J., and Chasin, L. A. (1978).Genetics 90:735–760.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Raskind, W.H., Gartier, S.M. X chromosome inactivation and SV40 transformation of mammalian cells. Somat Cell Mol Genet 5, 945–955 (1979). https://doi.org/10.1007/BF01542653
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01542653