Abstract
A new selective medium has been developed for cells containing the enzyme deoxycytidine deaminase. This medium contains hypoxanthine, aminopterin, and 5-methyldeoxycytidine (HAM medium). To survive in the presence of the aminopterin, the cells must utilize deoxycytidine deaminase to convert the 5-methyldeoxycytidine to thymidine. The cells must also have thymidine kinase and hypoxanthine phosphoribosyltransferase. A mouse cell line deficient in deoxycytidine deaminase has been isolated from a deoxycytidine kinase-deficient line, using 5-bromodeoxycytidine as the selective agent. A hybrid line between this double mutant and a human diploid fibroblast was isolated in HAM medium. The hybrid line contains the chromosomes expected of a human-mouse hybrid. The deoxycytidine deaminase isozyme patterns on cellogel show that the human-mouse hybrid cell line produces an enzyme with an electrophoretic mobility intermediate between that of the human and that of the mouse.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
Weiss, M. C., and Green, H. (1967).Proc. Natl. Acad. Sci. 58:1104–1111.
Green, H. (1969). In Defendi, V. (ed.),Wistar Institute Symposium Monograph No. 9, Wistar Institute Press, Philadelphia, pp. 51–59.
Ruddle, F. H. (1972). In Harris, H., and Hirschhorn, K. (eds.),Advances in Human Genetics, Vol. 3, Plenum Press, New York, pp. 173–235.
Szybalski, W., Szybalska, E. H., and Ragni, G. (1962).Natl. Cancer Inst. Monogr. 7:75–89.
Littlefield, J. W. (1964).Science 145:709–710.
Matsuya, Y., Green, H., and Basilico, C. (1968).Nature 220:1199–1202.
Migeon, B. R., and Miller, C. S. (1968).Science 162:1005–1006.
Miller, O. J., Allderdice, P. W., Miller, D. A., Breg, W. R., and Migeon, B. R. (1971).Science 173:244–245.
Miller, O. J., Cook, P. R., Meera Khan, P., Shin, S., and Siniscalco, M. (1971).Proc. Natl. Acad. Sci. 68:116–120.
Kusano, T., Long, C., and Green, H. (1971).Proc. Natl. Acad. Sci. 68:82–86.
Tischfield, J. A., and Ruddle, F. H. (1974).Proc. Natl. Acad. Sci. 71:45–49.
Bray, G. A. (1960).Anal. Biochem. 1:279–285.
Shin, S., Meera Khan, P., and Cook, P. R. (1971).Biochem. Genet. 5:91–99.
Todaro, G. J., and Green, H. (1963).J. Cell Biol. 17:299–313.
Chu, M. Y., and Fischer, G. A. (1965).Biochem. Pharmacol. 14:333–341.
Robert de Saint-Vincent, B., and Buttin, G. (1973).Europ. J. Biochem. 37:481–488.
Meuth, M., and Green, H. (1974).Cell,3:367–374.
Creasey, W. A. (1963).J. Biol. Chem. 238:1772–1776.
Cooper, G. M., and Greer, S. (1973).Mol. Pharmacol. 9:704–710.
Subak-Sharpe, J. H. (1969). In Wolstenholme, G. E. W., and Knight, J. (eds.),Ciba Foundation Symposium on Homeostatic Regulators, J. & A. Churchill, London, pp. 276–290.
Davidson, R. L., and Ephrussi, B. (1965).Nature 250:1170–1171.
Malathi, V. G., and Silber, R. (1971).Biochim. Biophys. Acta 238:377–387.
McFerran, N. V., Smyth, M., and Orsi, B. A. (1969).Biochem. J. 114:8P-9P.
Davidson, R., Ephrussi, B., and Yamamoto, K. (1968).J. Cell. Physiol. 72:115–128.
Weiss, M. C., and Ephrussi, B. (1966).Genetics 54:1111–1122.
Long, C., Chan, T., Levytska, V., Kusano, T., and Green, H. (1973).Biochem. Genet. 9:283–297.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chan, T.s., Long, C. & Green, H. A human-mouse somatic hybrid line selected for human deoxycytidine deaminase. Somat Cell Mol Genet 1, 81–90 (1975). https://doi.org/10.1007/BF01538733
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01538733