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Quantitative analysis of calcium gradients and activity in growing pollen tubes ofLilium longiflorum

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Summary

Pollen tubes ofLilium longiflorum were loaded with quin-2 to determine the cytoplasmic free calcium. Quin-2-fluorescence was detected at 500 nm with alternating excitation at 340 nm and 360 nm. The calcium2+-concentration was obtained using the intensity ratio R=I340/I360. The analysis exhibits a [Ca2+] of nearly 10−7mol·l−1 in the tip region and about 2·10−8 mol·l−1at the tube base, near the pollen grain. The data give evidence for the existence of a continuous gradient of free calcium within growing pollen tubes of various length.

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Authors and Affiliations

  1. Zellenlehre, Universität Heidelberg, Deutschland

    H. -D. Reiss

  2. I. Physiologisches Institut, Universität Heidelberg, Im Neuenheimer Feld 326, D-6900, Heidelberg, Germany

    R. Nobiling & H. -D. Reiss

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  1. R. Nobiling
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  2. H. -D. Reiss
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Nobiling, R., Reiss, H.D. Quantitative analysis of calcium gradients and activity in growing pollen tubes ofLilium longiflorum . Protoplasma 139, 20–24 (1987). https://doi.org/10.1007/BF01417531

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  • DOI: https://doi.org/10.1007/BF01417531

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