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Expression of hepatitis C virus core protein as a fusion protein with maltose binding protein

Detection of anti-hepatitis C core antibody by western blot

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Abstract

Putative hepatitis C virus core sequence was amplified from a serum sample positive for anti-C-100-3 and expressed inEscherichia coli. Approximately 62 kDa fusion protein with maltose binding protein containing 20 kDa hepatitis C core protein was obtained. The antibody to this protein was detected in 53 of 54 (98%) sera from hepatitis C virus ribonucleic acid-positive patients including 40 sera positive for anti-C-100-3 and 13 sera negative for anti-C-100-3. The antibody was also detected in all of 12 patients with acute hepatitis C showing the earlier detectability of the antibody than anti-C-100-3. Thus, the protein expressed from the amplified hepatitis C core sequence by the polymerase chain reaction would be useful for the diagnosis of hepatitis C.

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Authors and Affiliations

  1. First Department of Medicine, Chiba University School of Medicine, 1-8-1 Inohana, Chuou-ku, (260), Chiba, Japan

    Osamu Yokosuka MD &  Masao Omata MD

  2. Second Department of Medicine, Faculty of Medicine, Tokyo University, 7-3-1 Hongo, Bunkyo-ku, (113), Tokyo, Japan

    Osamu Yokosuka MD &  Masao Omata MD

Authors
  1. Osamu Yokosuka MD
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  2. Yoshimi Ito MD
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  3. Jun Sakuma MD
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  4. Fumio Imazeki MD
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  5. Masao Ohto MD
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  6. Masao Omata MD
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Yokosuka, O., Ito, Y., Sakuma, J. et al. Expression of hepatitis C virus core protein as a fusion protein with maltose binding protein. Digest Dis Sci 38, 626–630 (1993). https://doi.org/10.1007/BF01316791

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  • DOI: https://doi.org/10.1007/BF01316791

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