Summary
Two plaque types of Getah virus were isolated on BHK-21 cells from an emulsion of suckling mouse brain infected with strain AMM-2021 and were designated as large plaque (LP) and small plaque (SP) strains. Some of the properties of LP and SP strains were studied with the following results. The difference in plaque size of the LP and SP strains was quite distinct on BHK-21 cells and there was little difference in size at the 3rd—8th serial passage level in suckling mouse brain. The LP strain showed high hemagglutinating activity but the SP strain produced no detectable HA activity. The LP strain was shown to be more virulent than the SP strain to suckling mice by intracerebral or intraperitoneal inoculation. The LP strain was not neutralized as effectively as the SP strain with antisera to original, LP and SP strains. An enhancement of infectivity was observed when the LP strain was sensitized with either anti-LP or anti-SP hyperimmune mouse sera. This phenomenon was not pronounced with the SP strain.
Similar content being viewed by others
References
Brown, A.: Differences in maximum and minimum plaque-forming temperatures among selected group A arboviruses. Virology21, 362–372 (1963).
Clarke, D. H., Casals, J.: Technique for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Amer. J. trop. Med. Hyg.7, 561–573 (1958).
Hare, J. D., Morgan, H. R.: A polyoma virus variant with new antigenic determinants. Virology19, 105–107 (1962).
Hawkes, R. A.: Enhancement of the infectivity of arboviruses by specific antisera produced in domestic fowls. Aust. J. exp. Biol. med. Sci.42, 465–482 (1964).
Hearn, H. J., Jr., Brown, A., Hardy, F. M.: Alteration of virulence of Venezuelan equine encephalomyelitis virus after passage in L cell cultures and in mice. J. inf. Dis.108, 237–242 (1961).
Hearn, H. J., Jr., Soper, W. T.: Properties of Venezuelan equine encephalomyelitis virus populations. J. Virol.1, 453–459 (1967).
Inoue, K. Y., Nishibe, Y.: A small plaque mutants of Japanese encephalitis virus. Arch. ges. Virusforsch.15, 525–530 (1965).
Kimura, T., Iwai, K., Ueba, N.: Difference in plaque sizes of Japanese encephalitis virus strains on BHK21 cells and chick embryo cells. Biken J.16, 57–66 (1973).
Marshall, D., Scrivani, R. P., Reeves, W. C.: Variation in the size of plaques produced in tissue culture by strains of western equine encephalitis virus. Amer. J. Hyg.76, 225–238 (1962).
Mayer, V.: Two variants of tick-borne encephalitis virus showing different plaque morphology. Virology20, 372–373 (1963).
Nagai, K., Hammon, W. M.: Plaque studies with certain group B arboviruses. I. Japanese B encephalitis virus strains in hamster kidney and chick embryo tissue culture. Proc. Soc. exp. Biol. (N.Y.)117, 154–159 (1964).
Nagata, I., Kimura, Y., Matsumoto, T., Maeno, K., Yoshii, S., Nagai, Y., Iinuma, M.: Plaque variants of sindbis virus. Arch. ges. Virusforsch.22, 79–86 (1967).
Takemoto, K. K.: Plaque mutants of animal viruses. Prog. med. Virol.8, 314–348 (1966).
Ueba, N., Kimura, T.: Enhancement of the infectivity of Japanese encephalitis virus in BHK21 cells by specific antisera. (In Japanese.) Abstr. of papers, 21st Annual Meeting of Japanese Virologists, Virus 23, Article No. 75 (1973).
Author information
Authors and Affiliations
Additional information
With 2 Figures
Rights and permissions
About this article
Cite this article
Kimura, T., Ueba, N. Some biological and serological properties of large and small plaque variants of Getah virus. Archives of Virology 57, 221–229 (1978). https://doi.org/10.1007/BF01315086
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01315086