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Coxsackie B 1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness

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Summary

To analyze the possible mechanisms by which coxsackie B 1 virus infection affects the invasiveness ofShigella flexneri, we have studied the influence of intracellular levels of Na+ and K+, ATPase activity, cytoplasmic membrane potential, cAMP level and cell communication through gap junctions. 3 h after adsorption of viable or UV-inactivated coxsackie B 1 virus the Na+-K+ gradient of the cell collapsed, ATPase activity decreased, the cytoplasmic membranic potential-dependent tetraphosphonium ion uptake were reduced. No changes in cAMP or intercellular cell communication were observed.S. flexneri invasiveness in HEp-2 cell pretreated with viable or UV-inactivated coxsackie B 1 virus was enhanced, but bacterial invasiveness was unchanged in K+-depleted HEp-2 cells, cell cultures with high intracellular Na+ content or ouabain pre-treated cells compared to control cells. We found no correlation between the enhanced bacterial invasiveness in the early phase of coxsackie B 1 virus infection in HEp-2 cell cultures and intracellular K+ depletion, high intracellular Na+ content, inhibited Na+-K+ ATPase activity or membranic depolarization.

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Authors and Affiliations

  1. Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Oslo, Norway

    K. Modalsli, G. Bukholm & M. Degré

  2. Laboratory for Environmental and Occupational Cancer, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway

    S. O. Mikalsen

Authors
  1. K. Modalsli
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  2. G. Bukholm
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  3. S. O. Mikalsen
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  4. M. Degré
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Modalsli, K., Bukholm, G., Mikalsen, S.O. et al. Coxsackie B 1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness. Archives of Virology 124, 321–332 (1992). https://doi.org/10.1007/BF01309812

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  • DOI: https://doi.org/10.1007/BF01309812

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