Skip to main content
SpringerLink
Log in

Serological comparison of tospovirus isolates from Taiwan and India with impatiens necrotic spot virus and different tomato spotted wilt virus isolates

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Tospovirus isolates from tomato and watermelon in Taiwan and from peanut in India reacted neither with antibodies to tomato spotted wilt virus (TSWV), nor with antibodies to impatiens necrotic spot virus (INSV), in ELISA and in electroblot immunoassays. Additionally, all three tospovirus isolates had an N protein with a molecular weight of 32 kDa as compared to 29 kDa for the N protein to TSWV and INSV. The observed physicochemical and serological differences suggest the generation of a new, third species in the genus tospovirus for which we propose the name groundnut bud necrosis virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adam G, Chagas CM, Lesemann D-E (1987) Comparison of three rhabdovirus isolates by two different serological techniques. J Phytopathol 120: 31–43

    Google Scholar 

  2. Adam G, Lesemann D-E, Vetten J-H (1991) Monoclonal antibodies against tomato spotted wilt virus: characterization and application. Ann Appl Biol 118: 87–104

    Google Scholar 

  3. Cho JJ, Mau RFL, Hamasaki RT, Gonsalves D (1988) Detection of tomato spotted wilt virus in individual thrips by enzyme-linked immunosorbent assay. Phytopathology 78: 1348–1352

    Google Scholar 

  4. Clark MJ, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34: 574–586

    Google Scholar 

  5. De Avilá AC, Huguenot C, Resende R De O, Kitajima EW, Goldbach RW, Peters D (1990) Serological differentiation of 20 isolates of tomato spotted wilt virus. J Gen Virol 71: 2801–2807

    Google Scholar 

  6. De Avilá AC, De Haan P, Kitajima EW, Kormelink R, Resende R De O, Goldbach RW, Peters D (1992) Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) fromImpatiens sp. in the Netherlands. J Phytopathol 134: 133–151

    Google Scholar 

  7. De Haan P, Wagemakers L, Peters D, Goldbach WR (1990) The S RNA segment of tomato spotted wilt virus has an ambisense character. J Gen Virol 71: 1001–1007

    Google Scholar 

  8. De Haan P, Kormelink R, Resende R De O, Van Poelwijk F, Peters D, Goldbach RW (1991) Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. J Gen Virol 72: 2207–2216

    Google Scholar 

  9. Francki RIB, Fauquet CM, Knudson DL, Brown F (eds) (1991) Classification and nomenclature of viruses. Fifth report of the International Committee on Taxonomy of Viruses. Arch Virol [Suppl 2]

  10. Gonsalves D, Trujillo EE (1986) Tomato spotted wilt virus in papaya and detection of the virus by ELISA. Plant Dis 70: 501–506

    Google Scholar 

  11. Huguenot C, Van den Dobbelsteen G, De Haan P, Wagemakers CAM, Drost GA, Osterhaus ADME, Peters D (1990) Detection of tomato spotted wilt virus using monoclonal antibodies and riboprobes. Arch Virol 110: 47–62

    Google Scholar 

  12. Ie TS (1970) Tomato spotted wilt virus. CMI/AAB Description of Plant Virus, no 39

  13. Iwaki M, Honda Y, Hanada K, Tochihara H, Yanaha T, Hokama K, Yokoyama T (1984) Silver mottle disease of watermelon caused by tomato spotted wilt virus. Plant Dis 68: 1006–1008

    Google Scholar 

  14. Kitajima EW, De Avilá AC, Resende R De O, Goldbach RW, Peters D (1992) Comparative cytological and immunogold labelling studies on different isolates of tomato spotted wilt virus. J Submicrosc Cytol Pathol 24: 1–14

    Google Scholar 

  15. Laemmli UK (1972) Cleavage of the structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680–685

    Google Scholar 

  16. Law MD, Moyer JW (1990) A tomato spotted wilt-like virus with serologically distinct N protein. J Gen Virol 71: 933–938

    Google Scholar 

  17. Law MD, Speck J, Moyer JW (1991) Genomic organization of the impatiens necrotic spot virus M RNA. Phytopathology 81: 1192

    Google Scholar 

  18. Law MD, Speck J, Moyer JW (1991) Nucleotide sequence of the 3′ non-coding region and N gene of the S RNA of a serologically distinct tospovirus. J Gen Virol 72: 2579–2601

    Google Scholar 

  19. Law MD, Speck J, Moyer JW (1992) The M RNA of impatiens necrotic spot tospovirus (Bunyaviridae) has an ambisense genomic organization. Virology 188: 732–741

    Google Scholar 

  20. Maiss E, Ivanova L, Breyel E, Adam G (1991) Cloning and sequencing of the S-RNA from a Bulgarian tomato spotted wilt virus isolate. J Gen Virol 72: 461–464

    Google Scholar 

  21. Milne RG (1970) An electron microscope study of tomato spotted wilt virus in sections of infected cells and in negative stain preparations. J Gen Virol 6: 267–276

    Google Scholar 

  22. Wang M, Gonsalves D (1990) ELISA detection of various tomato spotted wilt virus isolates using specific antisera to structural proteins of the virus. Plant Dis 74: 154–158

    Google Scholar 

  23. Mohamed NA, Randles JW, Francki RIB (1973) Protein composition of tomato spotted wilt virus. Virology 56: 12–21

    Google Scholar 

  24. Peters D, De Avilá AC, Kitajima EW, Resende R De O, De Haan P, Goldbach RW (1990) An overview of tomato spotted wilt virus. In: Hsu H-T, Lawson RH (eds) Virus-thrips-plant interactions of tomato spotted wilt virus. Proceedings of a USDA Workshop, Beltsville, MD 1990. USDA Agricultural Research Service, National Technical Information Service, Springfield, pp 1–14

    Google Scholar 

  25. Pringle CR (1991) The 20th meeting of the executive committee of the International Committee on Virus Taxonomy. Virus species, higher taxa, a Universal Virus Database, and other matters. Arch Virol 119: 303–304

    Google Scholar 

  26. Reddy DVR, Ratna AS, Sudarshana MR, Poul F, Kiran Kumar I (1992) Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India. Ann Appl Biol 120: 279–286

    Google Scholar 

  27. Resende R De O, De Avilá AC, Goldbach RW, Peters D (1991) Comparison of polyclonal antisera in the detection of tomato spotted wilt virus using the double antibody sandwich and cocktail ELISA. J Phytopathol 132: 46–56

    Google Scholar 

  28. Sherwood JL, Sanborn MR, Keyser GC, Myers LD (1989) Use of monoclonal antibodies in detection of tomato spotted wilt virus. Phytopathology 79: 61–64

    Google Scholar 

  29. Sreenivasulu P, Demski JW, Reddy DVR, Naidu RA, Ratna AS (1991) Purification and some serological relationships of tomato spotted wilt virus isolates occurring on peanut (Arachis hypogaea) in the USA. Plant Pathol 40: 503–507

    Google Scholar 

  30. Tas PWL, Boerjan ML, Peters D (1977) The structural proteins of tomato spotted wilt virus. J Gen Virol 36: 267–279

    Google Scholar 

  31. Urban LA, Huang P-Y, Moyer JW (1991) Cytoplasmic inclusions in cells infected with isolates of L and I serogroups of tomato spotted wilt virus. Phytopathology 81: 25–529

    Google Scholar 

  32. Van Etten JL, Freer SN, McCune BK (1979) Presence of a major (storage?) protein in dormant spores of the fungusBotryodiplodia theobromae. J Bacteriol 138: 650–652

    Google Scholar 

  33. Van den Hurk J, Tas PWL, Peters D (1977) The ribonucleic acid of tomato spotted wilt virus. J Gen Virol 36: 81–91

    Google Scholar 

  34. Van Regenmortel MHV, Maniloff H, Calisher C (1991) The concept of virus species. Arch Virol 120: 313–314

    Google Scholar 

  35. Yeh S-H, Lin Y-C, Cheng Y-H, Jih C-L, Chen M-J, Chen C-C (1992) Identification of tomato spotted wilt-like virus on watermelon in Taiwan. Plant Dis 76: 835–840

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung für Pflanzenviren, Deutsche Sammlung von Mikroorganismen, Biologische Bundesanstalt für Land- und Forstwirtschaft, Braunschweig, Germany

    G. Adam

  2. Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan

    S. -D. Yeh

  3. International Crops Research Institute for Semi-Arid Tropics, Andhra Pradesh, India

    D. V. R. Reddy

  4. The Asian Vegetable Research and Development Center, Tainan, Taiwan

    S. K. Green

Authors
  1. G. Adam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. -D. Yeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. V. R. Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. K. Green
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Adam, G., Yeh, S.D., Reddy, D.V.R. et al. Serological comparison of tospovirus isolates from Taiwan and India with impatiens necrotic spot virus and different tomato spotted wilt virus isolates. Archives of Virology 130, 237–250 (1993). https://doi.org/10.1007/BF01309657

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01309657

Keywords

Search

Navigation