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Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection

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Summary

Stable neutralization and protection escape variants of a virulent strain (Trinidad Donkey) of the VEE virus were selected by monoclonal antibodies (MAbs). Determination of nucleotide sequences of nine variants revealed a clustering of single mutations in four regions of the E1 and E2 glycoproteins. Involvement of amino acid residues 206 (site E1-1), 57 and 59 (site E2-2), 180, 182, 213, 214 and 216 (site E2-6) and 232 (site E2-3) in protective epitopes was demonstrated.

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Author notes

  1. E. V. Agapov, I. V. Frolov & A. A. Kolykhalov

    Present address: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA

Authors and Affiliations

  1. Institute of Molecular Biology, State Research Center of Virology and Biotechnology "Vector", Koltsovo, Russia

    E. V. Agapov, I. A. Razumov, I. V. Frolov, A. A. Kolykhalov, S. V. Netesov & V. B. Loktev

Authors
  1. E. V. Agapov
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  2. I. A. Razumov
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  3. I. V. Frolov
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  4. A. A. Kolykhalov
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  5. S. V. Netesov
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  6. V. B. Loktev
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Agapov, E.V., Razumov, I.A., Frolov, I.V. et al. Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection. Archives of Virology 139, 173–181 (1994). https://doi.org/10.1007/BF01309462

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  • DOI: https://doi.org/10.1007/BF01309462

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