Summary
Changes in the structuredness,i.e. average microviscosity, of cytoplasm in yeast cells progressing from the stationary to the logarithmic phase of growth were investigated. The concomitant variations in the first order rate constant for the enzymatic hydrolysis of fluorescein diacetatein vivo were found to be inversely related to variations in the structure lness of cytoplasm. The hypothesis that the structuredness of cytoplasm participates in the control and regulation of rates of enzyme reactions is put forward.
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Cercek, L., Cercek, B.: Studies on the structuredness of cytoplasm and rates of enzymatic hydrolysis in growing yeast cells. I. Changes induced by ionizing radiation. Int. J. Radiat. Biol.21, 445–453 (1972).
Cercek, L., Cercek, B.: Studies on the structuredness of cytoplasm and rates of enzymatic hydrolysis in growing yeast cells. II. Changes induced by ultra-violet light. Int. J. Radiat. Biol.22, 539–544 (1972).
Cercek, L., Cercek, B.: Effect of centrifugal forces on the structuredness of cytoplasm in growing yeast cells. Biophysik9, 105–108 (1973).
Cercek, L.: Radiolytic changes in the structuredness of the cytoplasm as studiedin vivo. Curr. Top. Radiat. Res.7, 355–358 (1972).
Cercek, L.: Effects of 14 MeV neutrons and 300 kVp X-rays on geotropic response and sedimentation velocity of statoliths in barley roots. Int. J. Radiat. Biol.16, 419–429 (1969).
Glasstone, S., Laidler, K. J., Eyring, H.: The theory of rate processes. London and New York: McGraw-Hill Book Comp. Inc. 1941.
Guilbautt, G. G., Kramer, D. N.: Fluorometric determination of lipase, acylase, alpha- and gamma-chymotrypsin and inhibitors of these enzymes. Analyt. Chem.36, 409–412 (1964).
Wickerham, L. J.: Taxonomy of yeasts. Tech. Bull. U.AS. Deptmt. Agric. 1029 (1951).
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Cercek, L., Cercek, B. Relationship between changes in the structuredness of cytoplasm and rate constants for the hydrolysis of FDA inSaccharomyces cerevisiae . Biophysik 9, 109–112 (1973). https://doi.org/10.1007/BF01215849
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DOI: https://doi.org/10.1007/BF01215849