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Purification of alkaline protease of Rhizopus oryzae by foam fractionation

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Abstract

Studies on the cost effective purification method is very much essential for the industrially important enzyme like protease. As foam fractionation is an important technique in downstream processing of the biologicals, purification of the crude proteolytic enzyme produced by Rhizopus oryzae has been attempted using converging-diverging foam fractionator. The effects of different parameters studied on the purification efficiency by foam fractionation were pH of the broth, air flow rate, initial liquid height. The results obtained were found quite encouraging at pH 10 with 1 LPM of air flow rate at low initial liquid height.

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Authors and Affiliations

  1. Biotechnology unit Department of Chemical Engineering, Indian Institute of Technology Kharagpur, 721302, Kharagpur, India

    Rintu Banerjee, Rajeev Agnihotri & B. C. Bhattacharyya

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  1. Rintu Banerjee
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  2. Rajeev Agnihotri
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  3. B. C. Bhattacharyya
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Banerjee, R., Agnihotri, R. & Bhattacharyya, B.C. Purification of alkaline protease of Rhizopus oryzae by foam fractionation. Bioprocess Engineering 9, 245–248 (1993). https://doi.org/10.1007/BF01061529

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  • DOI: https://doi.org/10.1007/BF01061529

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