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Isolation of thermostable D-hydantoinase-producing thermophilicBacillus sp SD-1

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Summary

A thermophilic bacterium which showed highest thermostability and activity of the hydantoinase was isolated from 1m000 thermophiles and identified to beBacillus sp. SD-1 according to morphological and physiological characteristics. The optimal growth temperature of the bacterium was about 60°C. The hydantoinase ofBacillus sp. SD-1 was strictly D-specific, and optimal pH and temperature were determined to be about 8.0 and 70°C, respectively. The D-hydantoinase was stable upto 70°C, and half-life of the enzyme was about 20 min at 80°C.

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References

  • Bradford, M.M. (1976) Anal. Biochem. 72, 248–254

    Google Scholar 

  • Kim, D.M. and Kim, H.S. (1993) Enzyme Microb. Technol. 15, 530–534

    Google Scholar 

  • Kristjansson, J.K., Stetter, K.O. (1992) Thermophilic bacteria. In: Thermophillic bacteria, Kristjansson, J.K., eds. pp. 1–18, Boca Raton Ann Arbor London: CRC Press

    Google Scholar 

  • Morin, A., Hummel, W., Schutte, H., Kula, MR. (1986) Biotechnol. Appl. Biochem. 8, 564–574

    Google Scholar 

  • Morin, A., Leblanc, D., Paleczek, A., Hummel, W., Kula, M.R. (1990) J. Biotechnol. 16, 37–48

    Google Scholar 

  • Morin, A., Touzel, J.P., Lafond, A., Leblanc, D. (1991) Appl. Microbiol. Biotechnol. 35, 536–540

    Google Scholar 

  • Runser, S.M., Meyer, P.C. (1993) Eur. J. Biochem. 213, 1315–1324

    Google Scholar 

  • Suzuki, T., Igarashi, K., Hase, K., Tuzimura, K. (1973) Agri. Biol. Chem. 37, 411–416

    Google Scholar 

  • Syldatk, C., Laufer, A., Muller, R., Hoke, H. (1990) Production of optically pure D- and L-α-amino acids by bioconversion of D,L-5-monosubstituted hydantoin derivatives. In: Advances in Biochemical Engineering/Biotechnology, Fiechter, A., eds. vol. 41. pp. 29–95, Berlin Heidelberg: Springer-Verlag.

    Google Scholar 

  • Takahashi, S., Kii, Y., Kumagai, H., Yamada, H. (1978) J. Ferment. Technol. 56, 492–498

    Google Scholar 

  • Takahashi, S., Ohashi, T., Kii, Y., Kumagai, H., Yamada, H. (1979) J. Ferment. Technol. 57, 326–332

    Google Scholar 

  • Yamada, H., Shimizu, S., Shimada, H., Tani, Y., Takahashi, S., Ohashi, T. (1980) Biochimie 62, 395–399

    Google Scholar 

  • Yamada, H., Takahashi, S., Kii, Y., Kumagai, H. (1978) J. Ferment. Technol. 56, 484–491

    Google Scholar 

  • Yokozeki, K., Nakamori, S., Eguchi, C., Yamada, K., Mitsugi, K. (1987) Agri. Biol. Chem. 51, 355–362

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Korea Advanced Institute of Science and Technology, 373-1 Kusung-Dong, Yusung-Gu, 305-701, Taejon, Korea

    Seung-Goo Lee, Dong-Cheol Lee & Hak-Sung Kim

  2. Applied Microbiology Research Group, GERI, KIST, Yusung-Gu, P.O. Box 115, 305-600, Taejon, Korea

    Moon-Hee Sung

Authors
  1. Seung-Goo Lee
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  2. Dong-Cheol Lee
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  3. Moon-Hee Sung
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  4. Hak-Sung Kim
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Lee, SG., Lee, DC., Sung, MH. et al. Isolation of thermostable D-hydantoinase-producing thermophilicBacillus sp SD-1. Biotechnol Lett 16, 461–466 (1994). https://doi.org/10.1007/BF01023326

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