Abstract
We investigated the chemical basis for ants carrying the seeds ofHepatica americana, an ant-dispersed plant of eastern North America. A laboratory bioassay of seed and elaiosome extracts was based on the distance test items were carried byPogonomyrmex rugosus. Ants responded equally to isolated elaiosomes and to the diglyceride fraction. Diolein was a major component of the diglyceride fraction, which is consistent with a finding that 1,2-diolein releases seed-carrying byAphaenogaster rudis. Ants' response to the free fatty acid fraction was less intense. Gas chromatography of the fatty acid fraction indicated that oleic acid was a primary component, and oleic acid is known to elicit necrophoric behavior. No evidence supports an earlier suggestion that ricinoleic acid stimulates seed-carrying behavior. Ants failed to respond to seeds from which elaiosomes were removed.
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References
Beattie, A.J. 1985. The Evolutionary Ecology of Ant-Plant Mutualisms. Cambridge University Press, New York.
Bigley, W.S., andVinson, S.B. 1975. Characterization of a brood pheromone isolated from the sexual brood of the imported fire ant,Solenopsis invicta.Ann. Entomol. Soc. Am. 68:301–304.
Blum, M.S. 1970. The chemical basis of insect sociality, pp. 61–94,in M. Beroza (ed.). Chemicals Controlling Insect Behavior. Academic Press, New York.
Bradshaw, J.W.S., andHowse, P.E. 1984. Sociochemicals of ants, pp. 429–473,in W.J. Bell and R.T. Cardé (eds.). Chemical Ecology of Insects. Sinauer Associates, Sunderland, Massachusetts.
Bresinsky, A. 1963. Bau, Entwicklungsgeschichte und Inhaltsstoffe der Elaiosomen. Studien zur myrmekochoren Verbreitung von Samen und Fruchten. Bibliotheca Botanica Heft 126, E. Schweizerbart'sche Verlagsbuchhandlung, Stuttgart.
Bullock, S.H. 1974. Seed dispersal ofDentromecon by the seed predatorPogonomyrmex.Madrono 22:378–379.
Carroll, C.R., andJanzen, D.H. 1973. Ecology of foraging by ants.Annu. Rev. Ecol. Syst. 4:231–257.
Creighton, W.W. 1950. The Ants of North America. Cosmos Press, Cambridge, Massachusetts.
Davidson, D.W. 1977. Species diversity and community organization in desert seed-eating ants.Ecology 58:711–724.
Folch, I., Lees, M., andSloane-Stanley, G.H. 1957. A simple method for the isolation and purification of total lipids from animal tissues.J. Biol. Chem. 226:497–509.
Gordon, D.M. 1983. Dependence of necrophoric response to oleic acid on social context in the antPogonomyrmex. badius.J. Chem. Ecol. 9:105–111.
Hirsh, J., andAhrens, E.H. 1958. The separation of complex lipid mixtures by the use of silicic acid chromatography.J. Biol. Chem. 233:311–320.
Horvitz, C.C., andBeattie, A.J. 1980. Ant dispersal ofCalathea (Marantaceae) seeds by carnivorous ponerines (Formicidae) in a tropical rain forest.Am. J. Bot. 67:321–326.
Howard, D.F., andTschinkel, W.R. 1976. Aspects of nectrophoric behavior in the red fire ant,Solenopsis invicta.Behav. Ecol. Sociobiol. 56:157–179.
Kuskis, A. 1978. Handbook of Lipid Research. Plenum Press, New York.
Marshall, D.L., Beattie, A.J., andBollenbacher, W.E. 1979. Evidence for diglycerides as attractants in an ant-seed interaction.J. Chem. Ecol. 5:335–344.
Metcalfe, L.D., andSchmitz, A.A. 1961. The rapid preparation of fatty acid esters for gas chromatographic analysis.J. Anal. Chem. 33:363–364.
Motten, A.F. 1982. Autogamy and competition for pollinators inHepatica americana (Ranunculaceae).Am. J. Bot. 69:1296–1305.
O'Dowd, D.J., andHay, M.E. 1980. Mutualism between harvester ants and a desert ephemeral: Seed escape from rodents.Ecology 61:531–540.
Sernander, R. 1906. Entwurf einer Monographie der europaischen Myrmecochoren. K. Sven. Vetenskaps Akad. Handl. 41:1–410.
Simms, R.P.A., andLarose, J.A.G. 1962. The use of iodine vapor as a general detecting agent in the thin layer chromatography of lipids.J. Am. Oil Chemists' Soc. 39:232.
Skipski, V.P., andBarclay, M. 1968. Methods of Enzymology, Vol. XIV. John Wiley & Sons, New York.
Skipski, V.P., Smolowe, A.F., Sullivan, R.C., andBarclay, M. 1965. Separation of lipid classes by thin-layer chromatography.Biochim. Biophys. Acta 106:386–396.
Snelling, R.R., andGeorge, C.D. 1979. The Taxonomy, Distribution and Ecology of California Desert Ants. California Academy of Sciences, Los Angeles.
Sokal, R.R., andRohlf, F.J. 1981. Biometry. The Principles and Practice of Statistics in Biological Research. W.H. Freeman, San Francisco.
Vinson, S.B. 1972. Imported fire ant feeding onPaspalum seeds.Ann. Entomol. Soc. Am. 65:41.
Vinson, S.B., Thompson, J.L., andGreen, H.B. 1967. Phagostimulants for the imported fire antSolenopsis saevissima richerti.J. Insect Physiol. 13:1729–1736.
Wheeler, W.M. 1910. Ants: Their Structure, Development and Behavior. Columbia University Press, New York.
Wilson, E.O. 1971. The Insect Societies. Harvard University Press, Cambridge, Massachusetts.
Wilson, E.O., Durlach, N.I., andRoth, L.M. 1958. Chemical releasers of necrophoric behavior in ants.Psyche 65:108–114.
Wren, J.J. 1960. Chromatography of lipids on silicic acid.J. Chromatogr. 4:173–195.
Wren, J.J., andSzczepanowska, D. 1964. Chromatography of lipids in presence of an antioxidant, 4-methyl-2,6-di-tert-butylphenol.J. Chromatogr. 14:405–410.
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Skidmore, B.A., Heithaus, E.R. Lipid cues for seed-carrying by ants inHepatica americana . J Chem Ecol 14, 2185–2196 (1988). https://doi.org/10.1007/BF01014024
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DOI: https://doi.org/10.1007/BF01014024