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Localization of thyroid hormone in subpopulations of rat alveolar macrophages

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Summary

Radioimmunoassay and immunocytochemical staining methods were used to study the distribution of thyroid hormone in rat alveolar macrophages. The cells were fractionated into six subpopulations by Percoll density gradient. Positive immunoreactive tri-iodothyronine (T3) was observed in all subpopulations of macrophages, especially in low-density (1.040 and 1.050 g cm−3) groups, by avidin-biotin-peroxidase immunostaining techniques. The macrophages also showed various patterns of cellular T3 stainability. Results from radioimmunoassay of macrophage extracts also demonstrated that macrophages of low density had a higher level of total T3 than those of higher densities (≥1.060 g cm−3).

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Authors and Affiliations

  1. Department of Anatomy, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong

    W. K. Liu

  2. Department of Physiology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong

    C. C. Wong

  3. Department of Biology, Hong Kong Baptist College, Kowloon, Hong Kong

    N. K. Mak

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  1. W. K. Liu
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  2. C. C. Wong
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  3. N. K. Mak
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Liu, W.K., Wong, C.C. & Mak, N.K. Localization of thyroid hormone in subpopulations of rat alveolar macrophages. Histochem J 21, 115–121 (1989). https://doi.org/10.1007/BF01005987

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  • DOI: https://doi.org/10.1007/BF01005987

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