Summary
Two experiments were designed to test two alternative models of bacterial conjugation. According to the first model (Bouck andAdelberg, Nagata) the donor cell is ready for mating at the moment of completion of the chromosome replication.
According to the second model (Jacob andBrenner) a special replication cycle is started during mating and the new chromosome enters the recipient cell at the same moment it was synthesized. In the first experiment synchronization ofHfr cells was used. It was shown that the efficiency of mating is constant during the whole life cycle. In the second experiment a pulse of radioactive phosphate was introduced into the mating medium. The zygotes were frozen in liquid nitrogen and the radioactive “suicide” of recombinants measured. Only those genetic markers, which were transmitted simultaneously with the radioactive pulse underwent radioactive “suicide”. Both experiments are in accord with the second model of conjugation.
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Bouck, N., andE. A. Adelberg: The relationship between DNA synthesis and conjugation inEscherichia coli. Biochem. biophys. Res. Commun.11, 24 (1963).
Fisher, K. W.: The role of Krebs cycle in conjugation inEscherichia coli K 12. J. gen. Microbiol.16, 120 (1957).
Fraser, D., andE. A. Jerrel: The aminoacid composition of T3 bacteriophage. J. biol. Chem.205, 291 (1953).
Fuerst, C. R., andG. S. Stent: Inactivation ofEscherichia coli by decay of incorporated radioactive phosphorus. J. gen. Physiol. (Baltimore)40, 73 (1956).
Jacob, F., etS. Brenner: Sur la régulation de la synthèse du DNA chez les bactéries: l'hypothèse du réplicon. C. R. Acad. Sci., (Paris)256, 298 (1963).
——, andF. Cuzin: On the regulation of DNA replication in bacteria. Cold Spr. Harb. Symp. quant. Biol.28, 329 (1963).
— andE. L. Wollman: Sexuality and the genetics of bacteria. New York and London: Academic Press 1961.
Maaløe, O.: The control of normal DNA replication in bacteria. Cold Spr. Harb. Symp. quant. Biol.26, 45 (1961).
Maruyama, Y.: Physical methods for obtaining synchronous culture ofEscherichia coli. J. Bact.71, 542 (1956).
Nagata, T.: The molecular synchrony and sequential replication of DNA inEscherichia coli. Proc. nat. Acad. Sci. (Wash.)49, 551 (1963).
Starka, J., andJ. Koza: Nephelometric determination of cell count in synchronously dividing cultures of bacteria. Biochim. biophys. Acta (N. Y.)32, 261 (1953).
Stent, G. S., andC. R. Fuerst: Inactivation of bacteriophages by decay of incorporated radioactive phosphorus. J. gen. Microbiol.38, 441 (1955).
Suit, J. C., T. S. Matney, C. O. Doudney, andD. Billen: Transfer of theEscherichia coli K 12 chromosome in the absence of DNA synthesis. Biochem. biophys. Res. Commun.17, 237 (1964).
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Blinkova, A.A., Bresler, S.E. & Lanzov, V.A. DNA synthesis and chromosome transfer inEscherichia coli K 12 . Zeitschrift für Vererbungslehre 96, 267–274 (1965). https://doi.org/10.1007/BF00896826
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DOI: https://doi.org/10.1007/BF00896826