Summary
Interspecific hybrids have been obtained in an otherwise incompatible cross betweenBrassica juncea × Brassica hirta through the in vitro culture of hybrid ovules and ovaries. The best response was observed from ovules and ovaries cultured 10–15 and 5–7 days after pollination respectively on a basal medium supplemented with indoleacetic acid, kinetin and casein hydrolysate. In some cases the basal cut end of the ovaries proliferated to form callus and shoots. The in vitro-derived hybrid seeds varied in their colour, size and shape, and the F1 plants in the field showed a large diversity in their morphological traits. The hybrids were sterile, and had an intermediate number of chromosomes (2n=30).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
BajajY. P. S., 1986. Biotechnology in agriculture and forestry 2. Crops. I. Springer Verlag, Berlin-Heidelberg-New York-Tokyo.
BajajY. P. S., S. K.Mahajan & K. S.Labana, 1986. Interspecific hybridization ofBrassica napus andB. juncea through ovary, ovule and embryo culture. Euphytica 35: 103–109.
Downey, R. K., A. J. Klassen & G. R. Stringam, 1980. Rapeseed and mustard. p. 495–509. In:W. R. Fehr & H. H. Hadley (Eds), Hybridization of crop plants. Amer. Soc. Agron.
MohapatraD. & Y. P. S.Bajaj, 1984. In vitro hybridization in an incompatible cross —Brassica juncea × Brassica hirta. Curr. Sci. 53: 489–497.
MurashigeT. & F.Skoog, 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plantarum 15: 473–497.
WhiteP. R., 1963. The cultivation of animal and plant cells. The Ronald Press, New York.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mohapatra, D., Bajaj, Y.P.S. Interspecific hybridization inBrassica juncea × Brassica hirta using embryo rescue. Euphytica 36, 321–326 (1987). https://doi.org/10.1007/BF00730678
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00730678