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Lipid soluble weak organic acid buffers as “substrate” for pancreatic secretion

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Summary

The isolated cat pancreas was perfused with a Krebs-Henseleitsolution containing different concentrations of sulfamerazine buffer. Concomitantly the secretory rate, pH value, Cl− and total buffer concentration in the secretion were measured. The following were found:

  1. 1.

    The secretory rate dropped to very small values when the bicarbonate buffer was omitted from the perfusate.

  2. 2.

    Sulfamerazine can partly replace the CO2-bicarbonate buffer in promoting water and solute secretion in the saline-perfused preparation of the cat's pancreas.

  3. 3.

    The secretion of the sulfamerazine buffer depended exclusively on the concentration of its undissociated component in the perfusate.

It is concluded that a separation of H+/OH− ions takes place presumably at the luminal cell border and that the undissociated form of sulfamerazine can penetrate the cell by ‘nonionic diffusion’. In this way a source of H+ ions is provided which may be used either for H+ transport or for the buffering of OH− ions. The process of nonionic diffusion seems to be rate limiting for the buffer secretion, at least when sulfamerazine is offered.

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Authors and Affiliations

  1. Max-Planck-Institut für Biophysik, Kennedyallee 70, BRD-6000, Frankfurt a.M., Germany

    I. Schulz, F. Ströver & K. J. Ullrich

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  1. I. Schulz
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  3. K. J. Ullrich
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Schulz, I., Ströver, F. & Ullrich, K.J. Lipid soluble weak organic acid buffers as “substrate” for pancreatic secretion. Pflugers Arch. 323, 121–140 (1971). https://doi.org/10.1007/BF00586444

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