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Genetic and physical mapping of the natural resistance-associated macrophage protein 1 (NRAMP1) in chicken

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Abstract

The chicken natural resistance-associated macrophage protein 1 (NRAMP1) gene has been mapped by linkage analysis by use of a reference panel to develop the chicken molecular genetic linkage map and by fluorescence in situ hybridization. The chicken homolog of the murine Nramp1 gene was mapped to a linkage group located on Chromosome (Chr) 7q13, which includes three genes (CD28, NDUSF1, and EF1B) that have previously been mapped either to mouse Chr 1 or to human Chr 2q. Physical mapping by pulsed-field gel electrophoresis revealed that NRAMP1 is tightly linked to the villin gene and that the genomic organization (gene order and presence of CpG islands) of the chromosomal region carrying NRAMP1 is well conserved between the chicken and mammalian genomes. The regions on mouse Chr 1, human Chr 2q, and chicken Chr 7q that encompass NRAMP1 represent large conserved chromosomal segments between the mammalian and avian genomes. The chromosome mapping of the chicken NRAMP1 gene is a first step in determining its possible role in differential susceptibility to salmonellosis in this species.

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References

  • Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Neibergs, H.L., Zhang, N., Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teale, A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel, D.J.S. (1994). A genetic linkage map of the bovine genome. Nature Genet. 6, 227–235.

    Google Scholar 

  • Barrow, P.A., Huggins, M.B., Lovell, M.A., Simpson, J.M. (1987). Observations on the pathogenesis of experimental Salmonella typhimurium infection in chickens. Res. Vet. Sci. 42, 194–199.

    Google Scholar 

  • Bradley, D.J. (1977). Genetic control of Leishmania populations within the host. II. Genetic control of acute susceptibility of mice to L. donavani infections. Clin. Exp. Immunol. 30, 130–140.

    Google Scholar 

  • Bumstead, N., Barrow, P.A. (1988). Genetics of resistance to Salmonella typhimurium in newly hatched chicks. Br. Poultry Sci. 29, 521–529.

    Google Scholar 

  • Bumstead, N., Palyga, J. (1992). A preliminary linkage map of the chicken genome. Genomics 13, 690–697.

    Google Scholar 

  • Bumstead, N., Messer, L.I., Greenwood, N.G. (1987). Use of ev loci as a measure of inbreeding in domestic fowls. Br. Poultry Sci. 28, 717–725.

    Google Scholar 

  • Bumstead, N., Young, J.R., Tregaskes, C. (1993). TaqI and HaeIII RFLP at the locus encoding the chicken homologue of CD28. Anim. Genet. 24, 328.

    Google Scholar 

  • Bumstead, N., Wain, H., Salmon, N.S., Silliboume, J. (1995). Genomic mapping of immunological genes. In Advances in Avian Immunological Research, J.F. Davison, ed. Abingdon, UK: Carfax, in prees.

    Google Scholar 

  • Burt, D.W., Bumstead, N., Bitgood, J.J., Ponce de Leon, F.A., Crittenden, L.B. (1995). Chicken genome mapping: a new era in avian genetics. Trends Genet. 11, 190–194.

    Google Scholar 

  • Cellier, M., Govoni, G., Vidal, S., Kwan, T., Groulx, N., Liu, J., Sanchez, F., Skamene, E., Schurr, E., Gros, P. (1994). Human natural resistance-associated macrophage protein: cDNA cloning, chromosomal mapping, genomic organization, and tissue-specific expression. J. Exp. Med. 180, 1741–1752.

    Google Scholar 

  • Copeland, N.G., Jenkins, N.A., Gilbert, D.J., Eppig, J.T., Maltais, L.J., Miller, J.C., Dietrich, W.F., Weaver, A., Lincoln, S.E., Steen, R.G., Stein, L.D., Nadeau, J.H., Lander, E.S. (1993). A genetic linkage map of the mouse: current applications and future prospects. Science 262, 57–66

    Google Scholar 

  • Duncan, A.M.V., Chow, W., Robinson, B.H. (1992). Localization of the human 75-kDal Fe-S protein of NADH-coenzyme Q reductase gene (NDUFS1) to 2q33-q34. Cytogenet. Cell Genet. 60, 212–213.

    Google Scholar 

  • Goto, Y., Buschman E., Skamene, E. (1989). Regulation of host response to Mycobacterium intracellulare in vivo and in vitro by the Bcg gene. Immunogenetics 30, 218–221.

    Google Scholar 

  • Gros, P., Skamene, E., Forget, A. (1981). Genetic control of natural resistance to Mycobacterium bovis (BCG) in mice. J. Immunol. 127, 2417–2421.

    Google Scholar 

  • Gruenheid, S. Cellier, M., Vidal, S., Gros, P. (1995). Identification and characterization of a second mouse Nramp gene. Genomics 25, 514–525.

    Google Scholar 

  • Hedberg, C.W., David, M.J., White, K.E., MacDonald, K.L., Osterholm M.T. (1993). Role of egg consumption in sporadic Salmonella enteritidis and Salmonella typhimurium infections in Minnesota. J. Infect. Dis. 167, 107–111.

    Google Scholar 

  • Hibbs, J.B., Jr., Taintor, R.R., Vavrin, Z. (1987). Macrophage cytotoxicity: role for l-arginine deiminase and imino nitrogen oxidation to nitrite. Science 235, 473–476.

    Google Scholar 

  • Howard, T.A., Rochelle, J.M., Seldin, M.F. (1991). CD28 and Ctla-4, two related members of the Ig supergene family, are tightly linked on proximal mouse chromosome 1. Immunogenetics 33, 74–76.

    Google Scholar 

  • Lafage-Pochitaloff, M., Costello, R., Couez, D., Simonetti, J., Mannoni, P., Mawas, C., Olive, D. (1990). Human CD28 and CTLA-4 Ig superfamily genes are located on chromosome 2 at bands q33-q34. Immunogenetics 31, 198–201.

    Google Scholar 

  • Levin, I., Santangelo, L., Cheng, H., Crittenden, L.B., Dodgson, J.B. (1994). An autosomal genetic linkage map of the chicken. J. Hered. 85, 79–85.

    Google Scholar 

  • Liew, F.Y., Millott, S., Parkinson, C., Palmer, R.M.J., Moncada, S. (1990). Macrophage killing of Leishmania parasite in vivo is mediated by nitric oxide from l-arginine. J. Immunol. 144, 4794–4797.

    Google Scholar 

  • Lundin L.G. (1993). Evolution of the vertebrate genome as reflected in paralogous chromosomal regions in man and the house mouse. Genomics 16, 1–19.

    Google Scholar 

  • Malo, D., Skamene, E. (1994). Genetic control of host resistance to infection. Trends Genet. 10, 365–371.

    Google Scholar 

  • Malo, D., Vidal, S., Hu, J., Skamene, E., Gros, P. (1993a). High resolution linkage map in the vicinity of the host resistance locus Bcg. Genomics 16, 655–663.

    Google Scholar 

  • Malo, D., Vidal, S., Lieman, J.H., Ward, D.C., Gros, P. (1993b). Physical delineation of the minimal chromosomal segment encompassing the murine host resistance locus Bcg. Genomics 17, 667–675.

    Google Scholar 

  • Malo, D., Vogan, K., Vidal, S., Hu, J., Cellier, M., Schurr, E., Fuks, A., Bumstead, N., Morgan, K., Gros, P. (1994). Haplotype mapping and sequence analysis of the mouse Nramp gene predict susceptibility to infection with intracellular parasites. Genomics 23, 51–61.

    Google Scholar 

  • Manly, K.E. (1993). A Macintosh program for storage and analysis of experimental genetic mapping data. Mamm. Genome 4, 303–313.

    Google Scholar 

  • Mock, B., Kozak, C., Selden, M.F., Ruff, N., D'Hoostelaere, L., Szpirer, C., Levan, G., Sevanez, H., O'Brien, S., Banner, C. (1989). A glutaminase (gls) gene maps to mouse chromosome 1, rat chromosome 9 and human chromosome 2. Genomics 5, 291–297.

    Google Scholar 

  • Montagutelli, X. (1990). GENE-LINK—a program in PASCAL for backcross genetic linkage analysis. J. Hered. 81, 490–491.

    Google Scholar 

  • Nadeau, J.H., Taylor, B.A. (1984). Lengths of chromosomal segments conserved since divergence of man and mouse. Proc. Natl. Acad. Sci. USA 81, 814–818.

    Google Scholar 

  • O'Brien, A.D., Rosenstreich, D.L., Scher, I., Campbell, G.H., MacDermott, R.P., Formal, S.B. (1980). Genetic control of susceptibility to Salmonella typhimurium infection in mice: role of the Lps gene. J. Immunol. 124, 20–24.

    Google Scholar 

  • O'Brien, A.D., Taylor, B.A., Rosenstreich, D.L. (1984). Genetic control of natural resistance to Salmonella typhimurium in mice during the late phase of infection. J. Immunol. 133, 3313–3318.

    Google Scholar 

  • O'Brien, S., Womack, J., Lyons, L.A., Moore, K.J., Jenkins, N.A., Copeland, N.G. (1993). Anchored reference loci for comparative genome mapping in mammals. Nature Genet. 3, 103–112.

    Google Scholar 

  • Oloffson, B., Bernadi, G. (1976). Organization of nucleotide sequences in the chicken genome. Eur. J. Biochem. 130, 241–245.

    Google Scholar 

  • Plant, J.E., Glynn, A. (1976). Genetics of resistance to infection with Salmonella typhimurium in mice. J. Infect. Dis. 133, 72–78.

    Google Scholar 

  • Ponce de León, F.A., Li, Y., Weng, Z. (1992). Early and late replicative chromosomal banding patterns of Gallus domesticus. J. Hered. 82, 36–42.

    Google Scholar 

  • Rawlings, D.J., Saffran, D.C., Tsukasa, S., Largaespada, D.A., Grimaldi, J.C., Cohen, L., Mohr, R.N., Bazan, J.F., Howard, M., Copeland, N., Jenkins, N.A., Witte, O.N. (1993). Mutation of unique region of Bruton's tyrosine kinase in immunodeficient XID mice. Science 261, 358–361.

    Google Scholar 

  • Rohrer, G.A., Alexander, L.J., Keele, J.W., Smith, T.P., Beattie, C.W. (1994). A microsatellite linkage map of the porcine genome. Genetics 136, 231–245.

    Google Scholar 

  • Schurr, E., Skamene, E., Morgan, K., Chu, M.-L., Gros, P. (1990). Mapping of Col3al and Col6a3 to proximal murine chromosome 1 identifies conserved linkage of structural protein genes between murine chromosome 1 and human chromosome 2q. Genomics 8, 477–486.

    Google Scholar 

  • Skamene, E., Gros, P., Forget, A., Patel, P.J., Nesbitt, M. (1984). Regulation of resistance to leprosy by chromosome 1 locus in the mouse. Immunogenetics 19, 117–120.

    Google Scholar 

  • Spurr, N.K., Cox, S., Naylor, S. (1993). Report and abstracts of the Second International Workshop on Human Chromosome 2 Mapping. Cytogenet. Cell Genet. 64, 69–92.

    Google Scholar 

  • Tanaka, T., Akira, S., Yoshida, K., Umemoto, M., Yoneda, Y., Shirafuji, N., Fujiwara, H., Suematsu, S., Yoshida, N., Kishimoto, T. (1995). Targeted disruption of the NF-IL6 gene discloses its essential role in bacteria killing and tumor cytotoxicity by macrophages. Cell 80, 353–361.

    Google Scholar 

  • Thomas, J.D., Sideras, P., Smith, C.L.E., Vorechovsky, I., Chapman, V., Paul, W.E. (1993). Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes. Science 261, 355–358.

    Google Scholar 

  • Unkles, S.E., Hawker, K.L., Grieve, C., Campell, E.I., Montague, P., Kinghorn, J.R. (1991). crnA encodes a nitrate transporter in Aspergillus nidulans. Proc. Natl. Acad. Sci. USA 88, 204–208.

    Google Scholar 

  • Vidal, S., Malo, D., Vogan, K., Skamene, E., Gros, P. (1993). Natural resistance to infection with intracellular parasites: isolation of a candidate gene for Bcg. Cell 73, 469–485.

    Google Scholar 

  • Vidal, S., Belouchi, M., Cellier, M., Beatty, B., Gros, P. (1995a). Cloning and characterization of a second human NRAMP gene on Chromosome 12q13. Mamim. Genome 6, 224–230.

    Google Scholar 

  • Vidal, S., Tremblay, M., Govoni, G., Sebastiani, G., Malo, D., Olivier, M., Skamene E., Jothy, S., Gros, P. (1995b). The Ity/Lsh/Bcg locus: natural resistance to infection with intracellular parasites is abrogated by disruption of the Nrampl gene. J.Exp.Med, in press.

  • White, J.K., Shaw, M.-A., Barton, C.H., Cerretti, D.P., Williams, H., Mock, B.A., Carter, N.P., Peacock, C.S., Blackwell, J.M. (1994). Genetic and physical mapping of 2q35 in the region of the NRAMP and IL8R genes: identification of a polymorphic repeat in exon 2 of NRAMP. Genomics 24, 295–302.

    Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, McGill University, Montreal, Canada

    J. Hu, E. Skamene & D. Malo

  2. Department of Biochemistry, McGill University, Montreal, Canada

    P. Gros

  3. Department of Human Genetics, McGill University, Montreal, Canada

    D. Malo

  4. Institute for Animal Health, Compton, England, UK

    N. Bumstead

  5. Department of Veterinary and Animal Science, University of Massachusetts, Amherst, Massachusetts, USA

    D. Burke & F. A. Ponce de León

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  1. J. Hu
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  2. N. Bumstead
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  4. F. A. Ponce de León
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  5. E. Skamene
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  6. P. Gros
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  7. D. Malo
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Hu, J., Bumstead, N., Burke, D. et al. Genetic and physical mapping of the natural resistance-associated macrophage protein 1 (NRAMP1) in chicken. Mammalian Genome 6, 809–815 (1995). https://doi.org/10.1007/BF00539010

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