Abstract
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1.
Some properties of the open-tipped, uninsulated, pH-sensitive glass microelectrode were examined in several electrical experiments.
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2.
Based on these observations, technical and theoretical problems were considered for application to the pH measurement in small cells.
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3.
The intracellular pH, (pH)i, of the epithelial cell in rat duodenum measured was approximately 7.0. A reduction in (pH)i was apparent (about 0.3) with the addition of 20 mM-glucose to the bathing fluid.
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4.
It was concluded, that with certain limitations such uninsulated, open-tipped microelectrodes may be successfully utilized for intracellular pH measurements.
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Okada, Y., Inouye, A. pH-sensitive glass microelectrodes and intracellular pH measurements. Biophys. Struct. Mechanism 2, 21–30 (1976). https://doi.org/10.1007/BF00535650
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DOI: https://doi.org/10.1007/BF00535650