Abstract
Electrophoretic variation for an intestinal enzyme that cleaves L-leucyl-β-naphthylamide has been discovered among inbred mouse strains. Several strains including related strains C57BL/6J, C57BL/10J, C57BR/cdJ, C57L/J, and C58/J demonstrate an electrophoretic band of this enzyme that is absent in other strains and stocks thus far observed. The enzyme is tentatively being called leucine arylaminopeptidase (LAP) and the variant genetic locus Lap-1. The presence of the band is determined by an allele designated Lap-1 a. Homozygotes for the alternate allele, Lap-1 b, are without the band and heterozygotes are, under our electrophoretic conditions, indistinguishable from Lap-1 a homozygotes. Data from recombinant inbred lines and a B6 D2F1×DBA/2J backcross established linkage of Lap-1 to dilute (d) and supernatant malic enzyme (Mod-1) on chromosome 9 in the following order: Lap-1-d-Mod-1. The Lap-1 to d map distance was estimated to be 21.3±4.6 cM from backcross data and 8.1±4.8 cM from recombinant inbred lines.
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This research was supported by U.S. Atomic Energy Commission Contract AT(11-1)-3267, by NIH Research Grants GM 19656 and GM 18684 from the National Institute of General Medical Sciences, by Contracts NO1-ES4-2156 and NO1-ES4-2159 from the National Institute of Environmental Health Sciences, and in part by income from the Endowment Funds of The Jackson Laboratory. The Jackson Laboratory is fully accredited by the American Association for Accreditation of Laboratory Animal Care.
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Womack, J.E., Lynes, M.A. & Taylor, B.A. Genetic variation of an intestinal leucine arylaminopeptidase (Lap-1) in the mouse and its location on chromosome 9. Biochem Genet 13, 511–518 (1975). https://doi.org/10.1007/BF00484910
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DOI: https://doi.org/10.1007/BF00484910