Abstract
The polypeptide compositions of fraction I protein isolated from six collections of Nicotiana glauca and from ten cultivars of N. tabacum, as well as a polyploid series and a male sterile line, have been analyzed by isoelectric focusing in polyacrylamide gel slabs containing 8 m urea. Apart from the male sterile line, none of the plants showed any variation from the species pattern of polypeptides. Fraction I protein from the male sterile Burley 21 cultivar of N. tabacum contained the two small subunit polypeptides of N. tabacum and the three large subunit polypeptides of an Australian species, probably N. megalosiphon. This indicates a changed chloroplast genome in the male sterile line in comparison to the normal fertile N. tabacum.
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Chan, P. H., and Wildman, S. G. (1972). Chloroplast DNA codes for the primary structure of the large subunit of fraction I protein. Biochim. Biophys. Acta 277677.
Chan, P. H., Sakano, K., Singh, S., and Wildman, S. G. (1972). Crystalline fraction I protein: Preparation in large yield. Science 1761145.
Gray, J. C., Kung, S. D., Wildman, S. G., and Sheen, S. J. (1974). Origin of Nicotiana tabacum L. detected by polypeptide composition of fraction I protein. Nature 252226.
Kawashima, N. (1969). Comparative studies on fraction I protein from spinach and tobacco leaves. Plant Cell Physiol. 1031.
Kawashima, N., and Wildman, S. G. (1970). Fraction I protein. Ann. Rev. Plant Physiol. 21325.
Kawashima, N., and Wildman, S. G. (1972). Studies on fraction I protein IV: Mode of inheritance of primary structure in relation to whether chloroplast or nuclear DNA contains the code for a chloroplast protein. Biochim. Biophys. Acta 26242.
Kung, S. D., Sakano, K., and Wildman, S. G. (1974). Multiple peptide composition of the large and small subunits of Nicotiana tabacum fraction I protein ascertained by fingerprinting and electro-focusing. Biochim. Biophys. Acta 365138.
Kung, S. D., Gray, J. C., Wildman S. G., and Carlson, P. S. (1975). Polypeptide composition of fraction I protein from parasexual hybrid plants in the genus Nicotiana. Science 187353.
Nitsch, J. P., and Nitsch, C. (1969). Haploid plants from pollen grains. Science 16385.
Rutner, A. C. (1970). Estimation of the molecular weight of ribulose diphosphate carboxylase sub-units. Biochem. Biophys. Res. Commun. 39923.
Rutner, A. C., and Lane, M. D. (1967). Nonidentical subunits of ribulose diphosphate carboxylase. Biochem. Biophys. Res. Commun. 28531.
Sakano, K., Kung, S. D., and Wildman, S. G. (1974). Identification of several chloroplast DNA genes which code for the large subunit of Nicotiana fraction I proteins. Mol. Gen. Genet. 13091.
Wildman, S. G., Lu-Liao, C., and Wong-Staal, F. (1973). Maternal inheritance, cytology and macromolecular composition of defective chloroplasts in a variegated mutant of Nicotiana tabacum. Planta 113293.
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Supported by Research Grant AI-00536 from the National Institutes of Health, U.S. Public Health Service, and by Contract AT(04-3)-34, Project 8, from the Division of Biology and Medicine, U.S. Atomic Energy Commission.
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Chen, K., Kung, S.D., Gray, J.C. et al. Polypeptide composition of fraction I protein from Nicotiana glauca and from cultivars of Nicotiana tabacum, including a male sterile line. Biochem Genet 13, 771–778 (1975). https://doi.org/10.1007/BF00484408
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DOI: https://doi.org/10.1007/BF00484408