Skip to main content
SpringerLink
Log in

An immunohistochemical and electron-microscopic study of vascular endothelial cells in vocal fold polyps

  • Published:
Archives of oto-rhino-laryngology Aims and scope Submit manuscript

Summary

Gelatinous and telangiectatic types can be differentiated among the human vocal fold polyps. Telangiectatic polyps are characterized by eosinophilic deposits consisting of fibrin and cellular blood constituents. Labyrinthine vascular channels are characteristic of these polyps, and are partially or completely lined by a single layer of flat cells. Using electron microscopy and immunohistochemical stainings (antibodies against factor VIII-related antigen, Ulex europaeus I lectin, and antibodies against lysozyme), we found that the lining cells are true vascular endothelial cells and are not organizing histiocytic cells that are arranged in an endothelial-like pattern.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Allen HJ, Johson EAZ, Matta KL (1977) A comparison of the binding specificities of lectins from Ulex europaeus and Lotus tetragonolobus. Immunol Commun 6: 585–602

    Google Scholar 

  2. Borisch B, Möller P, Harms D (1983) Lektin Ulex Europaeus I als Marker in der Differentialdiagnose von Gefäßtumoren. Pathologe 4: 241–243

    Google Scholar 

  3. Burgdorf WHC, Mukai K, Rosai J (1981) Immunohistochemical identification of factor VIII-related antigen in endothelial cells of cutaneous lesions of alleged vascular nature. Am J Clin Pathol 75: 167–171

    Google Scholar 

  4. Debray H, Decout D, Strecker G, Spik G, Montreuil J (1981) Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins. Eur J Biochem 117:41–55

    Google Scholar 

  5. Frenzel H, Hort W (1975) Vergleichende experimentelle Untersuchungen über die Thrombenorganisation in Arterien und Venen. Basic Res Cardiol 70: 480–494

    Google Scholar 

  6. Frenzel H, Kleinsasser O, Hort W (1980) Licht- und elektronenmikroskopische Untersuchungen an Stimmlippenpolypen des Menschen. Virchows Arch [Pathol Anat] 389: 189–204

    Google Scholar 

  7. Freudenberg N, Riese KH (1976) Characterization of cells of the normal aortic endothelium of adult rats and changes due to endotoxin shock. I. Communication: light microscopy, autoradiography, DNA cytophotometry, and enzyme histochemistry. Beitr Pathol 159: 125–142

    Google Scholar 

  8. Freudenberg N, Riese KH, Freudenberg MA (1983) The vascular endothelial system. Fischer, Stuttgart New York

    Google Scholar 

  9. Ghani AR, Tibbs DJ (1962) Role of blood-borne cells in organization of mural thrombi. Br Med J II: 1244–1247

    Google Scholar 

  10. Goldstein IJ, Hayes CE (1978) The lectins: carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem 35: 127–340

    Google Scholar 

  11. Irninger W (1963) Histologische Altersbestimmung von Thrombosen und Embolien. Virchows Arch [Pathol Anat] 336: 220–237

    Google Scholar 

  12. Kleinsasser O (1982) Pathogenesis of vocal cord polyps. Ann Otol Rhinol Laryngol 91: 378–381

    Google Scholar 

  13. Mackenzie DC, Hackett M, Topozulu C, Tibbs DJ (1968) Origin of arterial prothesis lining from circulating blood cells. Arch Pathol 97: 879–885

    Google Scholar 

  14. Mason CY, Taylor CR (1975) The distribution of muramidase (Lysozyme) in human tissues. J Clin Pathol 28: 124–132

    Google Scholar 

  15. Miettinen M, Holthöfer H, Lehto VP, Miettinen A, Virtanen I (1983) Ulex europaeus I lectin as a marker for tumors derived from endothelial cells. Am J Clin Pathol 79: 32–36

    Google Scholar 

  16. Ogawa K, Suzuki Y, Saito S (1971) Histochemical study on the laryngeal polyp. J Otolaryngol Jpn 74: 406–407

    Google Scholar 

  17. Pinkus GS, Said JW (1977) Profile of intracytoplasmic lysozyme in normal tissues, myeloproliferative disorders, hairy cell leukemia, and other pathologic processes; an immunoperoxydase study of paraffin sections and smears. Am J Pathol 89: 351–366

    Google Scholar 

  18. Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. J Histochem Cytochem 18:315–333

    Google Scholar 

  19. Thorgeirsson G, Robertson AL Jr (1978) The vascular endothelium-pathobiologic significance. Am J Pathol 93: 803–848

    Google Scholar 

  20. Weber T, Seitz RJ, Liebert UG, Gallasch E, Wechsler W (1984) Affinity cytochemistry of vascular endothelia in brain tumours by biotinylated Ulex europaeus type I lectin (UEA I). Acta Neuropathol (Berl) (in press)

  21. Weibel ER, Palade GE (1964) New cytoplasmic components in arterial endothelia. J Cell Biol 23: 101–112

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Pathology, University of Düsseldorf, Federal Republic of Germany

    H. Frenzel, A. Schmitt-Gräff & R. J. Seitz

Authors
  1. H. Frenzel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Schmitt-Gräff
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. J. Seitz
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Professor Dr. W. Hort to his 60th birthday

Rights and permissions

Reprints and permissions

About this article

Cite this article

Frenzel, H., Schmitt-Gräff, A. & Seitz, R.J. An immunohistochemical and electron-microscopic study of vascular endothelial cells in vocal fold polyps. Arch Otorhinolaryngol 241, 271–278 (1985). https://doi.org/10.1007/BF00453700

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00453700

Key words

Search

Navigation