Abstract
Rinamycin interfered with DNA-polymerase I of Escherichia coli in cell-free systems more effectively than actinomycin C. Its inhibitory action could be relieved by increasing the concentration of polymerase. The rinamycin concentrations that caused effective inhibition of the RNA-polymerase reaction in vitro were approximately 10 times higher than those required to inhibit the DNA-polymerase reaction in vitro.
Derinamycin was as effective an inhibitor for the DNA-polymerase I reaction as actinomycin C, while having been stimulative for the RNA-polymerase reaction in cell-free systems of Escherichia coli. Its inhibitory action on DNA synthesis in vitro could be overcome by adding more DNA. In fact, spectral and antagonism studies suggested some sort of interaction between derinamycin and DNA.
Similar content being viewed by others
References
Falaschi, A., Kornberg, A.: Phleomycin, an inhibitor of DNA polymerase. Fed. Proc. 23, 940–945 (1964)
Friedman, P. A., Joel, P. B., Goldberg, I. H.: Interaction of kanchanomycin with nucleic acids. I. Physical properties of the complex. Biochemistry 8, 1535–1544 (1969)
Goldberg, I. H., Joel, P. B., Friedman, P. A.: Interaction of kanchanomycin with nucleic acids. III. Contrasts in the mechanisms of ribonucleic acid and deoxyribonucleic acid polymerase reactions. Biochemistry 9, 4421–4427 (1970)
Goudot, A.: Chemical structure of antimetabolites and antibiotic action. In: Advances in antimicrobial and antineoplastic chemotherapy. Proceedings of the 7th International Congress of Chemotherapy, M. Hejzlar, M. Semonsky, S. Masak, eds., pp. 863–864. München-Berlin-Wien: Urban & Schwarzenberg 1972
Hurwitz, J., Furth, J. J., Malamy, M., Alexander, M.: The role of deoxyribonucleic acid (DNA) in ribonucleic acid (RNA) synthesis. III. The inhibition of the enzymic synthesis of RNA and DNA by actinomycin D and proflavine. Proc. nat. Acad. Sci. (Wash.) 48, 1222–1230 (1962)
Joel, P. B., Goldberg, I. H.: Inhibition of RNA and DNA polymerases by hedamycin. Biochim. biophys. Acta (Amst.) 224, 361–370 (1970)
Kamiyama, M.: Mechanism of action of chromomycin A3. III. Binding of chromomycin A3 with DNA and physicochemical properties of the complex. J. Biochem. 63, 566–572 (1968)
Kersten, w., Kersten, H., Szybalski, W.: Physicochemical properties of complexes between deoxyribonucleic acid and antibiotics which affect ribonucleic acid synthesis (actinomycin, daunomycin, cinerubin, nogalamycin, chromomycin, mithramycin and olivomycin). Biochemistry 5, 236–244 (1966)
Kohn, K. W., Bono, V. H., Jr., Kann, h. E., Jr.: Anthramycin, a new type of DNA-inhibiting antibiotic: Reaction with DNA and effect on nucleic acid synthesis in mouse leukemia cells. Biochem. biophys. Acta (Amst.) 155, 121–129 (1968)
Koschel, K., Hartmann, G., Kersten, W., Kersten, H.: The action of chromomycin and some anthracycline antibiotics on the DNA-dependent nucleic acid synthesis. Biochem. Z. 344, 76–86 (1966)
Reich, E.: Actinomycin, correlation of structure and function of its complexes with purines and DNA. Science 143, 684–689 (1964)
Saeki, T., Umezawa, H., Tokieda-Fujishige, T., Hori, M.: Inhibition of ribonucleotide reductase. J. Antibiot. 27, 225–227 (1974)
Tanaka, N., Nagai, K., Yamaguchi, H., Umezawa, H.: Inhibition of RNA and DNA polymerase reactions by pluramycin A. Biochem. biophys. Res. Commun. 21, 328–332 (1965)
Uchida, K., Zähner, H.: Metabolic products of microorganisms 137. Rinamycin, a new inhibitor of RNA synthesis. J. Antibiot. 28, 185–193 (1975a)
Uchida, K., Zähner, H.: Metabolic products of microorganisms. 142. A new antibiotic derinamycin, inhibitor of DNA and RNA synthesis. J. Antibiot. 28, 266–273 (1975b)
Zähner, H., Maas, W. K.: Biology of antibiotics. Berlin-Heidelberg-New York: Springer 1972
Zimmer, C., Puschendorf, B., Grunicke, H., Chandra, P., Venner, H.: Influence of netropsin and distamycin A on the secondary structure and template activity of DNA. Europ. J. Biochem. 21, 269–278 (1971)
Author information
Authors and Affiliations
Additional information
154. Mitteilung: U. Dähn, H. Hagenmaier, H. Höhne, W. A. König, G. Wolf, H. Zähner: Nikkomycin, ein neuer Hemmstoff der Chitinsynthese bei Pilzen. Arch. Microbiol. 107, 143–160 (1976).
Rights and permissions
About this article
Cite this article
Uchida, K., Zähner, H. Metabolic products of microorganisms. Arch. Microbiol. 107, 161–166 (1976). https://doi.org/10.1007/BF00446835
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00446835