Abstract
The uptake of the phosphonate ion, the active breakdown product in plant tissues of the systemic anti-Oomycete compound Fosetyl-Al (aluminium tris-Oethylphosphonate), was investigated in two Phytophthora spp. of differential sensitivity. Uptake was due to the simultaneous operation of two transport systems, one of low affinity (high K m) and one of high affinity (low K m). The relative contribution of each transport system varied with the external concentration of phosphonate, suggesting that phosphonate was a potent regulator of both systems. Phosphate was a partial competitive inhibitor with respect to phosphonate. Phosphate competed with phosphonate for uptake with a K i of 105 μM for P. cryptogea and 68 μM for P. citrophthora. Uptake was sensitive to pH, showing a maximum at pH 5.0 to 5.5. P. cryptogea was more efficient in phosphonate uptake, although it was less sensitive to inhibition by phosphonate in vitro, than P. citrophthora. This implied that the selective activity of phosphonate was not due to differential rates of uptake of this oxyanion. These results were discussed in relation to the mode of action of phosphonate towards Oomycetes.
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Barchietto T, Saindrenan P, Bompeix G (1988) Uptake and utilisation of phosphonate ions by Phytophthora citrophthora and Nectria haematococca in relation to their selective toxicity. Pestic Sic 22:159–167
Beever RE, Burns DJW (1977) Adaptative changes in phosphate uptake by the fungus Neurospora crassa in response to phosphate supply. J Bacteriol 132:520–525
Beever RE, Burns DJW (1980) Phosphorus uptake, storage and utilization by fungi. Adv Bot Res 8:127–219
Bertrand A, Ducret J, Debourge JC, Horrière D (1977) Etude des propriétés d'une nouvelle famille de fongicides: les monoéthyl phosphites metalliques. Caractéristiques physico-chimiques et propriétés biologiques. Phytiatr Phytopharm 26:3–18
Bieleski RL, Ferguson IB (1983) Physiology and metabolism of phosphate and its compounds. In: Laüchly A, Bieleski RL (eds) Encyclopedia of plant physiology, New Series, vol 15. Springer, Berlin Heidelberg New York, pp 422–450
Blasco F, Ducet G, Azoulay E (1976) Mise en évidence de deux systèmes de transport du phosphate chez Candida tropicalis. Biochimie 58:351–357
Bompeix G, Saindrenan P (1984) In vitro antifungal activity of Fosetyl-Al and phosphorous acid on Phytophthora species. Fruits 39:777–786
Borst-Pauwels GWFH (1981) Ion transport in yeast. Biochim Biophys Acta 650:88–127
Burns DJW, Beever RE (1977) Kinetic characterization of the two phosphate uptake systems in the fungus Neurospora crassa. J Bacteriol 132:511–519
Casida LE Jr (1960) Microbial oxidation and utilization of orthophosphite during growth. J Bacteriol 80:237–241
Coffey MD, Bower LA (1984) In vitro variability among isolates of eight Phytophthora species in response to phosphorous acid. Phytopathology 74:738–742
Dekker J (1977) Resistance. In: Marsh RW (ed) Systemic fungicides (2nd ed). Longman, London, pp 176–197
Fenn ME, Coffey MD (1984) Studies on the in vitro and in vivo antifungal activity of Fosetyl-Al and phosphorous acid. Phytopathology 74:606–611
Goodman J, Rothstein A (1957) The active transport of phosphate into the yeast cell. J Gen Physiol 40:915–923
Guest DI (1984) Modification of defence responses in tobacco and capsicum following treatment with Fosetyl-Al (aluminium tris-O-ethyl phosphonate). Physiol Plant Pathol 25:125–134
Guest DI (1986) Evidence from light microscopy of living tissues that Fosetyl-Al modifies the defence response in tobacco seedlings following inoculation by Phytophthora nicotianae var. nicotianae. Physiol Mol Plant Pathol 29:251–261
Jeanjean R, Gaudin C, Blasco F (1972) Mécanisme d'adsorption de l'ion phosphate par les Chlorelles: influence du pH. C R Acad Sci (Paris) 275:1119–1121
Lowendorf HS, Bazinet GF, Slayman CW (1975) Phosphate transport in Neurospora. Derepression of a high-affinity transport system during phosphorus starvation. Biochim Biophys Acta 389:541–549
Malacinski GM, Konetzka WA (1966) Bacterial oxidation of orthophosphite. J. Bacteriol 91:578–582
Medveczky N, Rosenberg H (1971) Phosphate transport in Escherichia coli. Biochim Biophys Acta 241:494–506
Pipke R, Schulz A, Amrhein N (1987) Uptake of glyphosate by an Arthrobacter sp. Appl Environm Microbiol 53:974–978
Rebeille F, Bligny R, Douce R (1982) Regulation of Pi uptake by Acer pseudoplatanus cells. Arch Biochem Biophys 219:371–378
Rosenberg H, La Nauze JM (1968) The isolation of a mutant of Bacillus cereus deficient in phosphate uptake. Biochim Biophys Acta 156:381–388
Rosenberg H, Gerdes RG, Chegwidden K (1977) Two systems for the uptake of phosphate in Escherichia coli. J Bacteriol 131:505–511
Saindrenan P, Darakis G, Bompeix G (1985) Determination of ethyl phosphite, phosphite and phosphate in plant tissues by anionexchange high-performance liquid chromatography and gas chromatography. J Chromatogr 347:267–273
Saindrenan P, Barchietto T, Bompeix G (1987) Modification of elicitation of the defense responses with phosphonate in the Phytophthora cryptogea-Vigna unguiculata interaction. Proceedings, 1th congress of the French Phytopathological Society, Rennes, 20–22 November
Saindrenan P, Barchietto T, Avelino J, Bompeix G (1988) Effects of phosphite on phytoalexin accumulation in leaves of cowpea infected with Phytophthora cryptogea. Physiol Mol Plant Pathol 32:425–435
Segel IH (1975) Enzyme kinetics. John Wiley, Inc, New York London, pp 161–226
Surin BP, Cox GB, Rosenberg H (1987) Molecular studies on the phosphate-specific transport system of Escherichia coli. In: Torriani-Gorini A, Rothmans FG, Silver S, Wright A, Yagil E (eds) Phosphate metabolism and cellular regulation in microorganisms. American Society for Microbiology, Washington, DC, pp 145–149
Tamai Y, Toh-E A, Oshima Y (1985) Regulation of inorganic phosphate transport systems in Saccharomyces cerevisiae. J Bacteriol 164:964–968
Van Wazer JR (1958) Phosphorus and its compounds, vol 1, Chemistry. Interscience Publ, New York, pp 345–418
Williams DJ, Beach BGW, Horrière D, Maréchal G (1977) LS 74783, a new systemic fungicide with activity against phycomycete diseases. Proceedings, British Crop Protection Conf, Pests and Diseases (Croydon) 2:565–573
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Barchietto, T., Saindrenan, P. & Bompeix, G. Characterization of phosphonate uptake in two Phytophthora spp. and its inhibition by phosphate. Arch. Microbiol. 151, 54–58 (1988). https://doi.org/10.1007/BF00444669
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DOI: https://doi.org/10.1007/BF00444669