Abstract
Thirty seven strains of the Aspergillus flavus group isolated from animal mixed feeds have been screened for their ability to produce aflatoxins in yeast extract and sucrose (YES), aflatoxin producing ability (APA), and coconut agar medium (CAM) media. The concentration and detection of the aflatoxins by different methods is compared. Five known aflatoxin-positive and one aflatoxin-negative strains have been used as controls. Only 5 out of the 37 strains (13.5%) were aflatoxin-producers in YES medium. Of these five strains and the five known aflatoxin-positive strains, only three showed blue fluorescence in APA medium and four in CAM medium. Generally, the aflatoxin concentration in CAM medium was higher than in YES and APA media. Using the ‘agar-plug method’ and by direct spotting of the YES broth on TLC plates, some aflatoxin-producing strains were not detected.
Similar content being viewed by others
References
Davis ND, Diener UL, Eldridge DW. Production of aflatoxins B1 and G1 by A. flavus in a semisynthetic medium. Appl Microbiol 1966; 14:378–80.
Filtenborg O, Frisvad JC. A simple screening-method for toxigenic moulds in pure cultures. Lebensm Wiss Technol 1980; 13:128–30.
Gimeno A. Thin layer Chromatographic determination of aflatoxins, ochratoxins, sterigmatocystin, zealarenone, citrinin, T-2 toxin, diacetoxyscirpenol, penicillic acid, patulin and penitrem A. J Assoc Off Anal Chem 1979; 62:579–85.
Hara S, Fennel DI, Hesseltine CW. Aflatoxin-producing strains of Aspergillus flavus detected by fluorescence of agar medium under ultraviolet light. Appl Microbiol 1974; 27:1118–23.
Jesenska Z, Polster M, Matyasova J, Polakova L. Collaborative study of the effectiveness of the screening test on APA medium for aflatoxin producing aspergilli. Zbl Bakt Hyg I Abt Orig B 1980; 171:408–15.
Le Bars J. Contamination fongique et micotoxique d'aliments composés fabriqués à la Guadeloupe. Sci Aliments 1982; 2:61–6.
Lin MT, Dianese JC. A Coconut-agar medium for rapid detection of aflatoxin production by Aspergillus spp. Phytopathology 1976; 66:1466–69.
Moreno Romo MA, Suarez Fernandez G. Aflatoxinproducing potential of Aspergillus flavus strains isolated from Spanish poultry feeds. Mycopathologia 1986; 95:129–32.
Raper KB, Fennell DI. The genus Aspergillus. Baltimore: Williams & Wilkins Co, 1965.
Reddy TV, Viswanathan L, Venkitasubramanian TA. High aflatoxin production on a chemically defined medium. Appl Microbiol 1971; 22:393–6.
Sanchis V, Viñas I, Jimenez M, Hernandez E. Detección rápida de cepas de Aspergillus flavus productoras de aflatoxinas. Rev Agroquim Tecnol Aliment 1983; 54–9.
Shih CN, Marth EH. Some cultural conditions that control biosynthesis of lipid and aflatoxin by Aspergillus parasiticus. Appl Microbiol 1974; 27:452–6.
Torrey GS, Marth EH. Silica gel medium to detect moulds that produce aflatoxin. Appl Environ Microbiol 1976; 32:376–80.
Tsai WYJ, Lambert JD, Bullerman LB. Simplified method for microscale production and quantification of aflatoxin in broth. J Food Prot 1984; 47:526–9.
Wicklow DT, Shotwell OL, Adams GL. Use of aflatoxinproducing ability medium to distinguish aflatoxin-producing strains of Aspergillus flavus. Appl Environ Microbiol 1981; 41:697–9.
Yousef AE, Marth EH. Growth and synthesis of aflatoxin by Aspergillus parasiticus in the presence of sorbic acid. J Food Prot 1981; 44:736–41.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Abarca, M.L., Bragulat, M.R., Bruguera, M.T. et al. Comparison of some screening methods for aflatoxigenic moulds. Mycopathologia 104, 75–79 (1988). https://doi.org/10.1007/BF00436930
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00436930