Summary
A series of λ derivatives carrying tof mutations were tested for their ability to give rise to plasmid λ dv. Phages carrying tof mutations that distorted expression of the pRoR-tof-OP operon, were unable to produce λ dv. Phages carrying an altered tof gene, having only a moderate effect on the same operon, produced unstable λdv's. On the other hand, those tof mutants where only the expression of the pLoL-N-exo operon, but not that of the pRoR-tof-OP operon was affected, produced stable λdv's.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berg, D.: Genes from phage λ for λdv plasmids. Virology 62, 224–233 (1974)
Calef, E., Avitabile, A., Del Giudice, L., Marchelli, C., Menna, T., Neubauer, T., Solla, A.: The genetics of anti-immune phenotype of defective lambda. In: The bacteriophage lambda (A. D. Hershey ed.), p. 609–620. New York: Cold Spring Harbor Lab. 1971
Campbell, A.: Genetic structure. In: The bacteriophage lambda (A. D. Hershey ed.), p. 13–44. New York: Cold Spring Harbor Lab. 1971
Echols, H., Green, L., Oppenheim, A. B., Honigman, A.: Role of the cro gene in bacteriophage lambda development. J. molec. Biol. 80, 203–216 (1973)
Eisen, H., Brachet, P., Pereira da Silva, L., Jacob, F.: Regulation of repressor expression in λ. Proc. nat. Acad. Sci. (Wash.) 66, 855–862 (1970)
Fujii, S., Boriuchi, T.: Studies on lambda virulent mutants. I. Isolation and characterization of revertants from λvirC mutant. Molec. gen. Genet. 118, 287–294 (1972)
Hampacherova, M., Koutecka, E., Neubauer, A.: Two repressors in bacteriophage lambda. Molec. gen. Genet. 120, 133–137 (1973)
Matsubara, K.: Interference in phage growth by a resident plasmid λdv. I. The mode of interference. Virology 50, 713–726 (1972)
Matsubara, K.: Preparation of plasmid λdv from bacteriophage λ: Role of promoter-operator in the plasmid replicon. J. Virol. 13, 596–602 (1974)
Matsubara, K.: Geentic studies and regulation of a replicon of plasmid λdv. J. Molec. Biol. in press (1976)
Matsubara, K., Kaiser, A. D.: λdv: An autonomously replicating fragment. Cold Spr. Harb. Symp. quant. Biol. 33, 769–775 (1968)
Pero, J.: Location of the phage λ gene responsible for turn off λ-exonuclease synthesis. Virology 40, 65–71 (1970)
Takeda, Y., Matsubara, K., Ogata, K.: Regulation of early expression in bacteriophage lambda: Effect of tof mutation on strand-specific transcription. Virology, 65, 374–385 (1975)
Tomizawa, J.: Functional cooperation of genes O and P. In: The bacteriophage lambda (A. D. Hershey ed.), p. 769–776. New York: Cold Spring Harbor Lab. 1971
Author information
Authors and Affiliations
Additional information
Communicated by G. Bertani
Rights and permissions
About this article
Cite this article
Matsubara, K., Takeda, Y. Role of the tof gene in the production and perpetuation of the λdv plasmid. Molec. Gen. Genet. 142, 225–230 (1975). https://doi.org/10.1007/BF00425647
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00425647