Abstract
We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombination results preferentially in a plasmid that carries both the LEU2-d and the entire LEU2 gene. In addition we also found one plasmid with a tandem LEU2 insertion and one plasmid where the LEU2-d gene was replaced by the entire LEU2 gene.
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Valinger, R., Braus, G., Niederberger, P. et al. Cloning of the LEU2 gene of Saccharomyces cerevisiae by in vivo recombination. Arch. Microbiol. 152, 263–268 (1989). https://doi.org/10.1007/BF00409661
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DOI: https://doi.org/10.1007/BF00409661