Summary
The activities of several enzymes involved in the oxidation of ethanol and in the formation of oxaloacetate from acetate were compared in the yeasts Saccharomyces cerevisiae, Hansenula anomala and Rhodotorula glutinis grown on glucose and acetate, respectively. The most striking differences in the regulation of enzyme activities were found for alcohol dehydrogenase, acetaldehyde dehydrogenase, and malate dehydrogenase. The activities of the other enzymes tested behaved rather similar; in all three yeast species the enzymes of the glyoxylic acid by-pass showed the most extensive increase of activity in cells grown on acetate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Black, S.: Potassium-activated yeast aldehyde dehydrogenase. In: S. P. Colowick and N. O. Kaplan (Eds).): Methods in Enzymology, Vol. I, pp. 508–511. New York: Academic Press 1955.
Cannata, J. J. B., and A. O. M. Stoppani: Phosphopyruvate carboxylase from Bakers' Yeast. I. Isolation, Purification, and characterization. J. biol. Chem. 238, 1196–1207 (1963).
Kornberg, H. L.: Synthesis of cell constituents from acetate via the glyoxylate cycle. Proc. of the Fourth Int. Congress of Biochem., Vienna 1958. XIII, pp. 251–266. London, New York, Paris, Los Angeles: Pergamon Press 1959.
Ochoa, S.: “Malic” Enzyme. In: S. P. Colowick and N. O. Kaplan (Eds.): Methods in Enzymology, Vol. I, pp. 739–753. New York: Academic Press 1955.
Polakis, E. S., and W. Bartley: Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. Biochem. J. 97, 284–297 (1965).
Ruiz-Amil, M., G. de Torrontegui, E. Palacián, L. Catalina, and M. Losada: Properties and function of yeast pyruvate carboxylase. J. biol. Chem. 240, 3485–3492 (1965).
Tavlitzki, J.: Action de l'acriflavine sur les levures. III. Étude de la croissance des mutants «petite colonie«. Ann. Inst. Pasteur 76, 497–509 (1949).
Windisch, S.: Die hefeartigen Pilze. In: F. Reiff, R. Kautzmann, H. Lüers u. M. Lindemann (Hrsg.): Die Hefen, Vol. I, p. 151. Nürnberg: H. Carl, 1960.
Witt, I., R. Kronau u. H. Holzer: Repression von Alkoholdehydrogenase, Malatdehydrogenase, Isocitratlyase und Malatsynthese in Hefe durch Glucose. Biochim. biophys. Acta (Amst.) 118, 522–537 (1966a).
———: Isoenzyme der Malatdehydrogenase und ihre Regulation in Saccharomyces cerevisiae. Biochim. biophys. Acta (Amst.) 128, 63–73 (1966b).