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Phormidium J-1 bioflocculant: production and activity

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Abstract

Phormidium J-1, a benthic filamentous cyanobacterium, isolated from a drainage channel, was found to produce a high molecular weight polymer. This substance can flocculate bentonite particles from suspensions. At the stationary phase of growth the cells excreted this bioflocculant into the surrounding medium. Production was enhanced by reduction of the calcium content in the growth medium or by increasing its EDTA content. Above the isoelectric point (pH 3.5) the bioflocculant is negatively charged. The presence of minimal concentrations of divalent cations in the reaction mixture is required for its flocculating activity. The production of bioflocculant could be of great importance in clarification of turbid water bodies, thus allowing light penetration to the sediment/water interface. Bioflocculant production and excretion was not found in several other benthic cyanobacteria.

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References

  • Avnimelech Y, Troeger BW, Reed LW (1982) Mutual flocculation of algae and clays: evidence and implications. Science 216:63–65

    Google Scholar 

  • Bogorad LA (1975) Phycobiliproteins and complementary chromatic adaptation. Ann Rev Plant Physiol 26:369–401

    Article  Google Scholar 

  • Castenholtz RW (1982) Motility and taxis. In: Carr NG, Whitten BA (eds) The biology of blue-green algae. Blackwell Scientific Publications, Oxford London Edingburg Boston Melbourne

    Google Scholar 

  • Dugan PR (1975) Bioflocculation and the accumulation of chemicals by floc-forming organisms. National Technical Information Service. Springfield, Virginia, 22151

    Google Scholar 

  • Fattom A, Shilo M (1984) Hydrophobicity as an adhesion mechanism of benthic cyanobacteria. Appl Environ Microbiol 47:135–143

    Google Scholar 

  • Fenchel T, Staarup BJ (1971) Vertical distribution of photosynthetic pigments and the penetration of light in marine sediments. Oikos 22:172–182

    Google Scholar 

  • Fleer GJ, Lyklema J (1974) Polymer adsorption and its effect on the stability of hydrophobic colloids. II. The flocculation process as studied with the silver iodoe-polyvinyl alcohol system. J Coll Interface Sci 46:1–12

    Article  Google Scholar 

  • Harris RH, Mitchell R (1973) The roles of polymers in microbial aggregation. Ann Rev Microbiol 27:27–48

    Article  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  Google Scholar 

  • Lyklema J (1968) Principles of the stability of lyophobic colloidal dispersion in non aqueous media. Adv Coll Interface Sci 2:65–114

    Google Scholar 

  • Michaels AS (1954) Aggregation of suspension by polyelectrolytes. Ind Eng Chem 46:1485–1490

    Google Scholar 

  • Osmond DWJ, Vincent B, Waite FA (1975) Stearic stabilization: A reappraisal of current theory. Colloid and Polymer Sci 253:676–682

    Google Scholar 

  • Parfitt RL, Greenland DJ (1970) The adsorption of poly (ethylene glycols) on clay minerals. Clay Minerals 8:305–316

    Google Scholar 

  • Sarker N, Teat AS (1973) Coagulation of negatively charged colloids by anionic polyelectrolytes and metal ions. J Coll Interface Sci 43:370–381

    Article  Google Scholar 

  • Santoro T, Stotsky G (1967) Influence of cations on flocculation of clay minerals by microbial metabolites by the electrical sensing zone particle analyzer. Soil Sci Soc Am Proc 31:761–765

    Google Scholar 

  • Schopf JW (1974) Paleobiology of pre-cambrian: The age of bluegreen algae. In: Dobzhansky T, Hecht MK, Steene WC (eds) Evolutionary biology, vol 7. Plenum Press, New York, pp 1–43

    Google Scholar 

  • Trick CG, Anderson RJ, Gillam A, Harrison PJ (1983) Prorocentrin: An extracellular siderophore produced by the dinoflagellate Prorocentrum minimum. Science 219:306–308

    Google Scholar 

  • Theng BKG (1979) Formation and properties of clay-polymer complexes. Elsevier Scientific Publishing Company, Amsterdam Oxford New York

    Google Scholar 

  • Van Olphen H (1963) An introduction to clay colloid chemistry. John Wiley and Sons, New York

    Google Scholar 

  • Vincent B (1974) The effect of adsorbed polymers on dispersion ability. Adv Coll Interface Sci 4:193–277

    Article  Google Scholar 

  • Zur R (1979) Interaction between algae and inorganic suspended solids. MSc. Thesis. Technion, Haifa, Israel

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Dedicated to Prof. Dr. H.-G. Schlegel on the occasion of his 60th birthday

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Fattom, A., Shilo, M. Phormidium J-1 bioflocculant: production and activity. Arch. Microbiol. 139, 421–426 (1984). https://doi.org/10.1007/BF00408390

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  • DOI: https://doi.org/10.1007/BF00408390

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