Abstract
Chromosome number in the cells of the first division cycle in the root tip of Hordeum vulgare (2n=14) was apparently reduced from 2n to n by the chloramphenicol (CAP) treatments in early S, S late and/or early G 2 stages. Morphological observations and measurements of relative DNA content per cell indicated that such reduction was due to tight alignment of chromosomes in pairs. —It was supposed that homologous chromosomes are close together in the interphase nucleus, and their successive association up to mitotic prometaphase was induced by the CAP treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allen, N.S., Wilson, G.B., Powell, S.: Comparative effects of colchicine and sodium nucleate. On somatic chromosomes of Allium and Tradescantia. J. Hered. 41, 159–163 (1950)
Avivi, L., Feldman, M., Bushuk, W.: The mechanism of somatic association in common wheat, Triticum aestivum L. I. Suppression of somatic association by colchicine. Genetics 62, 745–752 (1969)
Boss, J.: II. Chromosome paring in anaphase. Exp. Cell Res. 7, 225–231 (1954)
Chauhan, K.P.S., Abel, W.O.: Evidence for the association of homologous chro- mosomes during premeiotic stages in Impatiens and Salvia. Chromosoma (Berl.) 25, 297–302 (1968)
Comings, D.E.: The rationale for an ordered arrangement of chromatin in the interphase nucleus. Amer. J. hum. Genet. 20, 440–460 (1968)
Dubuc, J.P., McGinnis, G.C.: Somatic association in Avena sativa L. Science 167, 999–1000 (1970)
Fedak, G., Helgason, S.B.: Somatic association of chromosomes in barley. Canad. J. Genet. Cytol. 12, 496–500 (1970)
Feldman, M.: The effect of chromosomes 5B, 5D, and 5A on chromosomal pairing in Triticum aestivum. Proc. nat. Acad. Sci. (Wash.) 55, 1447–1453 (1966)
Feldman, M., Mello-Sampayo, T., Sears, E.R.: Somatic association in Triticum aestivum. Proc. nat. Acad. Sci. (Wash.) 56, 1192–1199 (1966)
Gillois, M.M. de: Modele de l'induction de pseudo-méioses produisant des cellules haploïdes. C. R. Acad. Sci. (Paris) 268, 602–604 (1969).
Grell, S.M.: Cytological studies in Culex. I. Somatic reduction divisions. Genetics 31, 60–76 (1946)
Gutz, H.: Induction of mitotic segregation with p-fluorophenylalanine in Schizosaccharomyces pombe. J. Bact. 92, 1567–1568 (1966)
Huskins, C.L.: Segregation and reduction in somatic tissues. I. Initial observations on Allium cepa. J. Hered. 39, 311–325 (1948)
Kitani, Y.: Orientation, arrangement and association of somatic chromosomes. Jap. J. Genet. 38, 244–256 (1963)
Kumar, S., Natarajan, A.T.: Kinetics of two-break chromosome exchanges and the spatial arrangement of chromosome strands in interphase nucleus. Nature (Lond.) 209, 796–797 (1966)
Lhoas, P.: Mitotic haploidization by treatment of Aspergillus niger diploids with para-fluorophenylalanine. Nature (Lond.) 190, 744 (1961)
MacDonald, I.R., Bacon, J.S.D., Vaughan, D., Ellis, R.J.: The relation between ion absorption and protein synthesis in beet disks. J. exp. Bot. 17, 822–837 (1966)
Maguire, M.P.: Evidence for homologous pairing of chromosomes prior to meiotic prophase in maize. Chromosoma (Berl.) 21, 221–234 (1967)
Nicklas, R.B.: The chromosome cycle of a primitive cecidomyiid Mycophila speyeri. Chromosoma (Berl.) 11, 402–418 (1960)
Putrament, A.: On the mechanism of mitotic recombination in Aspergillus nidulans. II. Simultaneous recombination within two closely linked cistrons. Molec. gen. Genet. 100, 321–336 (1967)
Rao, R.N., Natarajan, A.T.: Somatic association in relation to chemically induced chromosome aberrations in Vicia faba. Genetics 57, 821–835 (1967)
Sax, K.: An analysis of X-ray induced chromosomal aberrations in Tradescantia. Genetics 25, 41–68 (1940)
Shaw, M.W., Cohen, M.W.: Chromosome exchanges in human leukocytes induced by mitomycin C. Genetics 51, 181–190 (1965)
Sibatani, A., Naora, H.: Enhancement of colour intensity in the histochemical Feulgen reaction: Method and quantitative estimation. Experientia (Basel) 8, 268–269 (1952)
Stern, H., Hotta, Y.: DNA synthesis in relation to chromosome pairing and chiasma formation. Genetics 61, Suppl. 27–39 (1969).
Storey, W.B.: Somatic reduction in cycads. Science 159, 648–650 (1968)
Wagenaar, E.B.: End-to-end chromosome attachments in mitotic interphase and their possible significance to meiotic pairing. Chromosoma (Berl.) 26, 410–426 (1969)
Yamamoto, K., Yamaguchi, H.: Radiation-induced chromosomal aberration in relation to DNA synthesis. J. Radiat. Res. (Tokyo) 7, 115–122 (1966)
Yoshida, H., Yamamoto, K., Yamaguchi, H.: Fragmentation and nondisjunction of barley chromosomes after the treatment of chloramphenicol and cycloheximide. Cytologia (Tokyo) 37, 697–707 (1972)
Zimmermann, F.K.: Induction of mitotic gene conversion by mutagens. Mutation Res. 11, 327–337 (1971)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yoshida, H., Yamaguchi, H. Arrangement and association of somatic chromosomes induced by chloramphenicol in barley. Chromosoma 43, 399–407 (1973). https://doi.org/10.1007/BF00406746
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00406746