Summary
Measurements are described of fusicoccin (FC)-stimulated H+ efflux in barley (Hordeum vulgare L.) roots when K+ and Na+ concentrations were varied. In low-salt roots H+ efflux was stimulated in both 5 mM KCl and NaCl. In salt-saturated roots H+ efflux was stimulated more effectively in KCl than in NaCl solution. The stimulation of H+ efflux thus is parallel with the selectivity of these different root preparations for K+ and Na+ and with estimates of permeability ratios (P Na/P K) determined from electrical measurements. It is suggested that the results support electrogenic coupling between FC-stimulated H+ efflux and cation uptake.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ballio, A., Graniti, A., Pocchiari, F., Silano, V.: Some effects of “Fusicoccin A” on tomato leaf tissues. Life Sci. 7, pt. II, 751–760 (1968)
Briggs, G. E.: The absorption of salts by plant tissue, considered as ionic interchange. Ann. Botany (London) 46, 301–322 (1932)
Briggs, G. E.: Rate of uptake of salts by plant cells in relation to an anion pump. J. Exptl. Botany 14, 191–197 (1963)
Briggs, G. E., Hope, A. B., Robertson, R. N.: Electrolytes and plant cells. Oxford, U.K.: Blackwell 1961
Cram, W. J., Laties, G. G.: The use of short-term and quasi-steady influx in estimating plasmalemma and tonoplast influx in barley root cells at various external and internal chloride concentrations. Australian. J. Biol. Sci. 24, 633–646 (1971)
Davis, R. F.: Photoinduced changes in electrical potentials and H+ activities of the chloroplast, cytoplasm, and vacuole of Phaeoceros laevis. In: Membrane transport in plants (Zimmermann, U., Dainty, J., eds.), p. 197–201. Berlin-Heidelberg-New York: Springer 1974
Epstein, E.: Dual pattern of ion absorption by plant cells and by plants. Nature 212, 1324–1327 (1966)
Hiatt, A. J.: Relationship of cell sap pH to organic acid change during ion uptake. Plant Physiol. 42, 294–298 (1967)
Hiatt, A. J., Hendricks, S. B.: The role of CO2 fixation in accumulation of ions by barley roots. Z. Pflanzenphysiol. 56, 220–232 (1967)
Jackson, P. C., Adams, H. R.: Cation-anion balance during potassium and sodium absorption by barley roots. J. Gen. Physiol. 46, 369–386 (1963)
Jeschke, W. D.: K+-stimulated Na+-efflux and selective transport in barley roots. In: Ion Transport in Plants (Anderson, W. P., ed.), p. 285–296. London-New York: Acad. Press 1973
Läuchli, A., Epstein, E.: Transport of potassium and rubidium in plant roots: the significance of calcium. Plant Physiol. 45, 639–641 (1970)
Leigh, R. A., Wyn Jones, R. G.: The effect of increased internal ion concentration upon the ion uptake isotherms of excised maize root segments. J. Exptl. Botany 24, 787–795 (1973)
Marrè, E., Lado, P., Ferroni, A., Ballarin Denti, A.: Transmembrane potential increase induced by auxin, benzyladenine and fusicoccin. Correlation with proton extrusion and cell enlargement. Plant Sci. Lett. 2, 257–265 (1974)
Marschner, H., Schimansky, Ch.: Suitability of using rubidium-86 as a tracer for potassium in studying potassium uptake by barley plants. Z. Pflanzenernaehr. Düng. Bodenk. 128, 129–143 (1971)
Pitman, M. G.: Simulation of Cl- uptake by low-salt barley roots as a test of models of salt uptake. Plant Physiol. 45, 1417–1427 (1969)
Pitman, M. G.: Active H+ efflux from cells of low-salt barley roots during salt accumulation. Plant Physiol. 45, 787–790 (1970)
Pitman, M. G.: Uptake and transport of ions in barley seedlings. I. Estimation of chloride fluxes in cells of excised roots. Australian. J. Biol. Sci. 24, 407–421 (1971)
Pitman, M. G.: Uptake and transport of ions in barley seedlings. II. Evidence for two active stages in transport to the shoot. Australian. J. Biol. Sci. 25, 243–257 (1972)
Pitman, M. G., Courtice, A. C., Lee, Barbara: Comparison of potassium and sodium uptake by barley roots at high and low salt status. Australian. J. Biol. Sci. 21, 871–881 (1968)
Pitman, M. G., Mertz, S. M., Jr., Graves, J. S., Pierce, W. S., Higinbotham, N.: Electrical potential differences in cells of barley roots and their relation to ion uptake. Plant Physiol. 47, 76–80 (1970)
Pitman, M. G., Saddler, H. W.: Active sodium and potassium transport in cells of barley roots. Prov. Nat. Acad. Sci. US 57, 44–52 (1967)
Pitman, M. G., Schaefer, N., Wildes, R. A.: Stimulation of H+ efflux and cation uptake by fusicoccin in barley roots. Plant Sci. Lett. 4, 323–329 (1975)
Rains, D. W., Epstein, E.: Sodium absorption by barley roots: role of the dual mechanisms of alkali cation transport. Plant Physiol. 42, 314–318 (1967)
Scott, B. I. H., Gulline, Heather, Pallaghy, C. K.: The electrochemical state of cells of broad bean roots. I. Investigations of elongating roots of young seedlings. Australian. J. Biol. Sci. 21, 185–200 (1968)
Spear, D. G., Barr, J. K., Barr, C. E.: Localization of hydrogen ion and chloride ion fluxes of Nitella. J. Gen. Physiol. 54, 397–414 (1969)
Walker, N. A.: Membrane transport: theoretical background. In: Encycl. Plant Physiol., New Series vol. 2, (Lüttge, U., Pitman, M. G., eds.). Berlin-Heidelberg-New York: Springer 1975 (in press)
Walker, N. A., Pitman, M. G.: The measurement of fluxes across membranes. In: Encycl. Plant Physiol., New Series vol. 2, (Lüttge, U., Pitman, M. G., eds.). Berlin-Heidelberg-New York: Springer 1975 (in press)
Walker, N. A., Smith, F. A.: Intracellular pH in Chara corallina measured by DMO distribution. Plant Sci. Lett. 4, 125–132 (1975)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pitman, M.G., Schaefer, N. & Wildes, R.A. Relation between permeability to potassium and sodium ions and fusicoccin-stimulated hydrogen-ion efflux in barley roots. Planta 126, 61–73 (1975). https://doi.org/10.1007/BF00389360
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00389360