Summary
14CO2 fixation and radioactive products in Chlorella cells were examined during the induction period. Chlorella vulgaris (strain 211-11f) grown under low CO2 concentration (0.03 vol.-%) was used in our experiments. No transients in 14CO2 fixation could be observed under the conditions used (0.7 vol.-% CO2). Autoradiographic studies of the kinetics of appearance of labelled products showed that at the beginning of the illumination period radioactivity appeared first in intermediates of the Calvin cycle (e.g. phosphate esters). After 2 min smaller amounts of radioactivity were incorporated in malate and aspartate. In another series of experiments the influence of iodoacetamide (0.001 M) on 14CO2 fixation was examined. In Chlorella cells inhibited with iodoacetamide radioactivity was located in malate, aspartate and 3-phosphoglycerate only. Results were discussed with reference to the participation of two different carboxylation pathways in the formation of the transients. Also the possible role of photorespiration in these transients was discussed.
Similar content being viewed by others
Literatur
Aufdemgarten, H.: Zur Kenntnis der sogenannten Induktionsvorgänge bei der Kohlensäureassimilation. Planta (Berb) 29, 643–678 (1939).
Butler, L.: A secondary photosynthetic carboxylation. Plant Physiol. 35, 233–237 (1960).
Calo, N., Gibbs, M.: The site of inhibition of iodoacetamide in photosynthesis studied with chloroplasts and cell free preparations of spinach. Z. Naturforsch. 15b, 287–291 (1960).
Döhler, G.: Die Induktionseffekte der photosynthetischen CO2-Aufnahme von einzelligen Grünalgen sowie der Blaualge Anacystis und ihre deutung mit der Hypothese von zwei verschiedenen Carboxylierungsreaktionen. Habil.-Schr. Frankfurt a. M. 1968.
Döhler, G.: Transients in photosynthetic CO2 uptake of Chlorella and Anacystis. In: H. Metzner (ed.), Progress in photosynthesis research, vol. I, p. 531. München 1969.
Egle, K., Döhler, G.: Über die Induktionserscheinungen der Photosynthese und der Dunkelatmung bei einzelligen Grünalgen. Beitr. Biol. Pfl. 39, 295–322 (1963).
——: Die Wirkung von O2-Entzug auf die Induktionsphase der photosynthetischen CO2-Aufnahme. Z. Naturforsch. 19b, 773–774 (1964).
Gibbs, M., Kandler, O.: Asymmetric distribution of 14C in sugars formed during photosynthesis. Proc. nat. Acad. Sci. (Wash.) 43, 446–451 (1957).
Hatch, M. D., Slack, C. R.: Photosynthesis by sugar cane leaves. A new carboxylation reaction and the pathway of sugar formation. Biochem. J. 101, 103–111 (1966).
Kortschak, H. P., Harrt, C. E., Burr, C. O.: Carbon dioxide fixation in sugar cane leaves. Plant Physiol. 40, 209–213 (1965).
Schürmann, P.: Separation of phosphate esters and algal extracts by thin-layer electrophoresis and chromatography. J. Chromatogr. 39, 507–509 (1969).
Soeder, J. C., Ried, A.: Über den Verlauf von Protoplastenteilung und Autosporenfreisetzung in Synchronkulturen von Chlorella pyrenoidosa. Arch. Mikrobiol. 42, 173–189 (1962).
Vejlby, K.: Fotosynthesens primaere induktionsfaenomen. Thesis, Kobenhavn 1961.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Döhler, G., Wegmann, K. Untersuchungen zur Photosynthese-Induktion bei Chlorella mit Hilfe von radioaktivem CO2 . Planta 89, 266–274 (1969). https://doi.org/10.1007/BF00385031
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00385031