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Biochemical responses during starvation and subsequent recovery in postlarval Pacific white shrimp, Penaeus vannamei

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Abstract

Postlarval shrimp, Penaeus vannamei Boone, 1931, were held individually in cages and exposed to two feeding regimes. One group was starved for 12 d and then fed during the following 12 d. A second group was fed throughout the 24 d study. Four individuals were sampled from each of the two groups on Days 0, 1, 2, 4, 8, 12, 13, 14, 16, 20, and 24. Molting and growth among the starved-fed postlarvae stopped after 2 d starvation, while fed postlarvae increased significantly in size throughout the 24 d study. Among the starved-fed postlarvae, water content increased rapidly in response to starvation. DNA and sterol concentrations increased significantly during starvation due to selective catabolism of cellular components. After 12 d, RNA concentration was not significantly different between the fed and starved-fed postlarvae, but became significantly higher in the starved-fed postlarvae 48 h after feeding resumed. Triacylglycerol reserves were severely depleted during the first day of starvation, while protein concentrations began to decrease after the second day of starvation. RNA, protein, and the polyamines spermidine and spermine, when expressed as a ratio to DNA, decreased in response to starvation. Concentrations of all measured parameters in the starved-fed postlarvae returned to levels similar to those in the fed group 8 to 12 d after feeding resumed. Results of this study suggest that triacylglycerol provides energy during short periods of starvation, while protein is utilized during prolonged starvation. The ratios of RNA:DNA, protein:DNA, spermidine:DNA, spermine:DNA, two unidentified amine compounds, and percent water content are all useful indicators of prolonged nutritional stress in postlarval P. vannamei.

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Authors and Affiliations

  1. Department of Biology, University of Alabama at Birmingham, 35294, Birmingham, Alabama, USA

    K. C. Stuck & S. A. Watts

  2. Department of Biological Sciences, University of Southern Mississippi, 39406-5018, Hattiesburg, Mississippi, USA

    S. Y. Wang

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  1. K. C. Stuck
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  2. S. A. Watts
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  3. S. Y. Wang
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Communicated by N.H. Marcus, Tallahassee

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Stuck, K.C., Watts, S.A. & Wang, S.Y. Biochemical responses during starvation and subsequent recovery in postlarval Pacific white shrimp, Penaeus vannamei . Marine Biology 125, 33–45 (1996). https://doi.org/10.1007/BF00350758

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