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RNA:DNA ratio during the critical period and early larval growth of the red drum Sciaenops ocellatus

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Abstract

Eggs from two separate spawning stocks of the red drum Sciaenops ocellatus (Linnaeus) were hatched, and the larvae were reared in the laboratory for 2 wk under closely controlled conditions. Total RNA, DNA, and soluble protein were measured in each population daily in triplicate pooled samples of larvae from each of three tanks. Growth rate in mm d-1 was determined for each population at 2 d intervals. Growth rate explained 72 and 95% of the variation in the RNA:DNA ratios of the two populations individually, and 86% of the variation in the RNA:DNA ratio when data from the two populations were combined. The RNA:DNA ratio appeared to be most effective as an indicator of growth in rapidly growing larvae, and to lose some resolution when growth was intermittent. The rates of deposition of RNA, DNA, and protein into tissue were all highly correlated with growth rate and with each other. Mean population RNA:DNA ratios of red drum yolk-sac larvae decreased from Day 1 post-hatch until larvae initiated successful feeding behavior, and then increased steadily throghout the remainder of the experimental period. This pattern of change in the RNA:DNA ratios during the yolk-sac stage appears to be an intrinsic developmental pattern of red drum ontogeny. The lowest values for the RNA:DNA ratio were observed just prior to the initiation of feeding or during the “critical period”, indicating that red drum larvae experience a decrease in capacity for protein synthesis as they initiate feeding. Intrinsic variation in the RNA:DNA ratio during development suggests that caution be used when comparing the RNA:DNA ratios of yolk-sac larvae to a “critical ratio” calculated from Buckley's general model.

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  1. M. Westerman

    Present address: Department of Wildlife and Fisheries, Texas A&M University, 210 Nagle Hall, 77843, College Station, Texas, USA

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  1. Marine Science Institute, The University of Texas at Austin, P.O. Box 1267, 78373, Port Aransas, Texas, USA

    M. Westerman & G. J. Holt

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  1. M. Westerman
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  2. G. J. Holt
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Communicated by N. H. Marcus, Tallahassee

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Westerman, M., Holt, G.J. RNA:DNA ratio during the critical period and early larval growth of the red drum Sciaenops ocellatus . Marine Biology 121, 1–9 (1994). https://doi.org/10.1007/BF00349468

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