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Storage of monoamines in mast cells of normal and sectioned peripheral nerve

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Summary

A study was made on the storage of monoamines in mast cells of normal and sectioned peripheral nerve in the rat. The following principal findings were made:

  1. 1.

    In the sciatic nerve of normal rats mast cells in the epineurial connective tissue and those between the nerve fibres in the endoneurium contained cytoplasmic granules, which emitted a strong yellow fluorescence after formaldehyde gas treatment of freeze-dried tissues according to the method of Falck (1962).

  2. 2.

    After sectioning a large increase in the number of mast cells occurred throughout the distal degenerating part of the nerve. These mast cells contained intensely yellow fluorescent cytoplasmic granules.

  3. 3.

    The secondary fluorescence obtained in mast cells of normal and sectioned rat sciatic nerve was abolished by reduction of the fluorescent compounds with sodium borohydride according to Corrodi, Hillarp and Jonsson (1964) and could be regenerated with a renewed formaldehyde gas treatment.

The secondary fluorescence in mast cells of normal and sectioned rat sciatic nerve is thus typical for a monoamine of the tryptamine group and similar to that obtained from other rat mast cells known to store serotonin (5-hydroxytryptamine).

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Authors and Affiliations

  1. Neuropathological Laboratory, Department of Pathology I, University of Göteborg, Sweden

    Yngve Olsson

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  1. Yngve Olsson
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Acknowledgements. I want to express my profound gratitude to Assoc. Prof. Patrick Sourander for his support and valuable criticism of this work and to Prof. Arvid Carlsson Department of Pharmacology, University of Göteborg for placing the resources of his Department at my disposal and for reading the manuscript. I also want to express my sincere thanks to Assoc. Prof. Bengt Falck, Department of Histology University of Lund, who kindly thaught me the fluorescence method used in this investigation, Mr. George Thieme for advice and help in handling his freeze-drying apparatus and Miss Kristina Müntzing for skilful technical assistance.

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Olsson, Y. Storage of monoamines in mast cells of normal and sectioned peripheral nerve. Zeitschrift für Zellforschung 68, 255–265 (1965). https://doi.org/10.1007/BF00342432

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